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当前位置: 首页 > 文章 > 具铁载体活性植物病原真菌的筛选鉴定 云南农业大学学报(自然科学) 2020 (2) 235-242
Position: Home > Articles > Screening and Identification of Secretory Siderophore from Plant Pathogen Journal of Yunnan Agricultural University(Natural Science) 2020 (2) 235-242

具铁载体活性植物病原真菌的筛选鉴定

作  者：
何翔;张庆;李楚;李铭刚;徐胜涛;陈齐斌;杨佩文
单  位：
关键词：
植物病原真菌;铁载体;CAS检测法
摘  要：
【目的】从不同宿主植物病原真菌中筛选高产铁载体的菌种资源,并利用螯合性铁载体及病原菌对Fe3+的竞争机制,为病害防控提供新的作用靶点。【方法】采用CAS检测法和光吸收法进行分泌型铁载体产生菌的定性、定量筛选及类型的初步鉴定,并基于18S rDNA对高活性优势菌株进行生物学鉴定。【结果】经定性初筛和定量复筛,确定5株供试病原真菌为高产铁载体优势菌株,分泌铁载体类型为异羟肟酸型、羧酸盐型和儿茶酚型;经分子生物学鉴定,菌株TZT-024与烟曲霉(Aspergillus fumigatus)具较高同源性,菌株TZT-033与灰葡萄孢(Botrytis cinerea)具较高同源性,菌株TZT-037与刀孢蜡蚧菌(Lecanicillium psalliotae)具较高同源性,菌株TZT-047与吡喃腐霉(Pythium pyrilobum)具较高同源性,菌株TZT-069与韦司梅拟盘多毛孢(Pestalotiopsis vismiae)具较高同源性。【结论】目前,尚未有L. psalliotae、P. pyrilobum和P. vismiae等植物病原真菌分泌铁载体的研究报道,可作为后续开展铁载体防控病害研究的优选材料。
译  名：
Screening and Identification of Secretory Siderophore from Plant Pathogen
作  者：
HE Xiang;ZHANG Qing;LI Chu;LI Minggang;XU Shengtao;CHEN Qibin;YANG Peiwen;College of Plant Protection, Yunnan Agricultural University;Institute of Agricultural Environmental Resources, Yunnan Academy of Agricultural Sciences;Institute of Microbiology, Yunnan University;
关键词：
pathogenic fungi;;siderophore;;CAS assay
摘  要：
[Purpose]The strain resources of high-yield siderophore were screened from different host plant pathogenic fungi, and using high-sorghum siderophore and pathogens to compete with Fe3+can provide a new target for the control of plant diseases.[Methods]We qualitatively and quantitatively screen the pathogenic strains of different host plants and the preliminary identification of siderophore types by CAS method and light absorption method, and biological identification of the dominant strains was according to 18 S rDNA.[Results]There were 5 strains were identified as high-yield siderophore dominant strains from the test bacterial by the qualitative and quantitative screening, and the type of secretory siderophore are Hydroxamates, Carboxylates and Catecholates.According to the molecular biology identification, strain TZT-024 has similarity with Aspergillus fumigatus, strain TZT-033 is very similar to Botrytis cinerea, strain TZT-037 is analogous to Lecanicillium psalliotae, strain TZT-047 and TZT-069 respectively resemble Pythium pyrilobum and Pestalotiopsis vismiae.[ Conclusion] There are no relevant researches of siderophore production about L. psalliotae, P. pyrilobum and P. vismiae, currently. Therefore, it can be used as a preferred material for the disease prevention by siderophore.

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