Position: Home > Articles > Applied Study of Earthworm Molecular Biomarkers in Risk Assessment of Soil Chloroaniline Contamination
Journal of Agro-Environment Science
2010,29
(08)
1490-1494
蚯蚓分子生物标志物在土壤氯代苯胺类污染评价中的应用研究
作 者：
王晓尉;张波
单 位：
首都师范大学生命科学学院;北京联合大学应用文理学院
关键词：
DNA加合;DNA交联率;Western-Blot;3,4-二氯苯胺;蚯蚓
摘 要：
采用紫外分光光度法测定DNA加合物的形成及Western-Blot技术检测蚯蚓热休克蛋白70(HSP70)的表达,研究了蚯蚓DNA加合物和HSP70作为分子生物标志物在土壤氯代苯胺化合物污染评价中的应用。DNA加合实验的结果表明,随着3,4-二氯苯胺浓度的增加(0.77、1.55、3.10 mg.mL-1),特征吸收峰的位移发生显著改变,交联率呈现负相关;Western-Blot检测结果表明,3,4-二氯苯胺处理后蚯蚓HSP70表达增加,与空白组比较有显著性差异。
译 名：
Applied Study of Earthworm Molecular Biomarkers in Risk Assessment of Soil Chloroaniline Contamination
作 者：
WANG Xiao-wei1,ZHANG Bo2(1.College of Life Science,Capital Normal University,Beijing 100048,China;2.College of Applied Sciences and Humanities,Beijing Union University,Beijing 100191,China)
关键词：
DNA adduct;DNA rate of cross-linking;Western-Blot;3,4-dichloroaniline;earthworm
摘 要：
To study the effects of 3,4-dichloroaniline on earthworm DNA adducts and heat shock protein(HSP)70,which were used as biomarkers in risk assessment of soil chloroaniline contamination.DNA adducts was detected with UV spectrophotometry and HSP70 was measured with Western-Blot methods.The results of DNA adduct and rate of cross-linking showed that characteristic absorption peak signif-icantly shifts and rate of cross-linking presents a negative correlation with 3,4-dichloro-aniline concentration increased(0.77,1.55,3.10 mg.mL-1).The results of Western-Blot test showed that earthworms treated with 3,4-dichloroaniline had a high expression of HSP70 com-paring with the control group.
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