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当前位置: 首页 > 文章 > 白粉病菌入侵对不同抗性南瓜品种的病理和生理影响 安徽农业科学 2007,35 (6) 149-151+193
Position: Home > Articles > Changes of Histopathology and Isozymes in Susceptible and Resistant Pumpkin Cultivars Penetrated by Podosphaera xanthii Journal of Anhui Agricultural Sciences 2007,35 (6) 149-151+193

白粉病菌入侵对不同抗性南瓜品种的病理和生理影响

作  者：
周威;李彩霞;王飞;许晓风;石志琦
单  位：
江苏省农业科学院食品安全与检测研究所;南京师范大学生命科学学院;沈阳农业大学植物保护学院
关键词：
抗性鉴定;南瓜白粉病;H2O2;同工酶
摘  要：
为了寻找简便有效的品种鉴定方法,采用透明染色法比较了不同抗性南瓜品种接种白粉病菌(Podosphaera xanthii)后的组织病理表现,并用电泳分离法分析了这些品种染病前后过氧化物酶(POD)、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)的同工酶谱变化。结果显示:在抗病南瓜品种上,白粉病菌生长缓慢,分生孢子形成较晚,叶片受侵染部位H2O2生成较多;接种后,所有抗病南瓜品种POD和PPO同工酶谱带均显著增强,而感病南瓜品种POD和PPO同工酶谱带无明显变化。因此,在筛选抗白粉病的南瓜品种时,可以把接种后POD和PPO酶活激发与否作为鉴别是否为抗病品种的依据之一。
译  名：
Changes of Histopathology and Isozymes in Susceptible and Resistant Pumpkin Cultivars Penetrated by Podosphaera xanthii
作  者：
ZHOU Wei et al(College of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210097)
关键词：
Resistance identification;Pumpkin powdery mildew;H2O2;Isozymes
摘  要：
Screening of resistant varieties and application of resistant cultivars are necessary and available for crop protection.Identification of resistant pumpkin cultivars was limited by traditional screening method.To accelerate the speed of identification and find a new screening method,various pumpkin cultivars with different resistant levels to Podosphaera xanthii were studied on the histopathology by staining assays in this investigation.Isozymes of peroxidase(POD),polyphenol oxidase(PPO) and superoxide dismutase(SOD) in healthy and inoculated cultivars were analyzed by native-PAGE.Results showed that mycelial growth and conidial formation of Podosphaera xanthii were inhibited on resistant pumpkin cultivars.Moreover,H2O2 burst occurred in resistant cultivars at interaction sites 24 hours after inoculation.The intensity of POD and PPO isozyme bands in resistant cultivars was greatly enhanced at 48 hours after inoculation, while in susceptible cultivar these isozyme bands had no changes after inoculation. This research indicated that resistant pumpkin varieties could be simply identified by the enhancement of POD and PPO isozyme bands after inoculation.

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