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Position: Home > Articles > Content Determination of Aspirin Eugenol Ester Chewable Tablets by HPLC Progress in Veterinary Medicine 2019 (10) 48-53

HPLC法测定阿司匹林丁香酚酯在咀嚼片中的含量

作  者：
焦钰婷;焦增华;刘希望;杨亚军;杨孝朴;李剑勇
单  位：
甘肃农业大学动物医学院;农业部兽用药物创制重点实验室
关键词：
阿司匹林丁香酚酯;高效液相色谱法;咀嚼片;含量测定
摘  要：
建立阿司匹林丁香酚酯(AEE)咀嚼片中AEE含量测定的高效液相色谱法(HPLC)。样品经甲醇溶解后,利用Hypersil ODS(4.6 mm×150 mm,5μm)色谱柱分离,流动相为甲醇∶水(77∶23),流速为0.9 mL/min,紫外检测波长为279 nm,柱温为35℃,进样量10μL。AEE在1.0μg～80.0μg范围内与峰面积呈良好的线性关系(R~2=0.999 8),平均回收率为100.1%,RSD为1.21%(n=9)。结果表明,所建立的HPLC法快速简便、准确可靠,可用于AEE咀嚼片中AEE含量的测定。
译  名：
Content Determination of Aspirin Eugenol Ester Chewable Tablets by HPLC
作  者：
JIAO Yu-ting;JIAO Zeng-hua;LIU Xi-wang;YANG Ya-jun;YANG Xiao-pu;LI Jian-yong;College of Veterinary Medicine,Gansu Agricultural University;Key Lab of New Animal Drug Project of Gansu Province,Key Lab of Veterinary Pharmaceutical Development,Ministry of Agriculture,Lanzhou Institute of Animal Husbandry and Pharmaceutical Science of CAAS;
关键词：
yak;;Proteus mirabilis;;isolation and identification
摘  要：
In order to study the cause of yak death in a Gansu breeding farm,the strain was isolated from Yak lung tissue,cultured and identified by Gram staining and 16 S rRNA gene sequence analysis.The antimicrobial susceptibility test and mouse virulence test were conducted.The results showed that the strain was Gram-negative bacillus and migrated on TSA medium.It was difficult to form a single colony.The opaque colony with black spots was formed on SS medium.The results of 16 S rRNA sequencing of the isolate after Blast with reference strains show 99.6%-99.9% homology,and it was identified as Proteus mirabilis.According to the results of antimicrobial susceptibility test,the isolate was sensitive to 11 kinds of antimicrobials commonly used in clinic,and resistant to 6 kinds of drugs.The results provided the basis for clinical medication.Virulence tests confirmed that LD_(50) of the isolate to mice was 0.73×10~9 CFU.

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