关键词：
虎龙斑;微生态制剂;体成分;消化酶;组织结构
摘 要：
试验旨在探讨两种微生态制剂对虎龙斑体成分、肠道消化酶和组织学结构的影响.选用540尾体质量为(33.98±4.91)g的虎龙斑,随机分为6个组,在基础饲料中添加低浓度0.1%的芽孢杆菌制剂、高浓度0.5%的芽孢杆菌制剂、低浓度1%的光合细菌制剂和高浓度5%的光合细菌制剂,并以2%饲料用量的乳化鱼油包裹,配制成编号为Diet 1、Diet 2、Diet 3、Diet 4四种饲料作为微生态制剂添加组,再增设乳化鱼油组和对照组,试验期为60 d.试验结果表明:(1)微生态制剂可以显著提高虎龙斑粗蛋白质含量(P<0.05),降低粗脂肪含量.(2)微生态制剂可以提高虎龙斑肠道胰蛋白酶、淀粉酶活性,而脂肪酶活性则没有显著性差异(P>0.05).(3)在前肠的肠绒毛高度中,Diet 2组和Diet 3组显著高于乳化鱼油组(P<0.05),而在中肠中,Diet 2组的肠绒毛高度显著高于乳化鱼油组(P<0.05),肌层厚度和浆膜层在前肠、中肠和后肠中各组之间的高度没有显著性差异(P>0.05).(4)微生态制剂在一定程度上可以改变虎龙斑鲜肝脏的颜色,减少肝细胞的坏死和脂肪空泡化.(5)与对照组和乳化鱼油组相比,微生态制剂能增加虎龙斑头肾淋巴细胞、黑色素巨噬细胞的数量,而对头肾细胞总数和中性粒细胞没有显著性差异(P>0.05).结果表明饲料中添加微生态制剂在一定程度上可以提高鱼的肠道消化酶活性,改善鱼的肠道、肝脏和头肾结构,从而提高鱼的免疫力.
作 者：
Huang Ling;Li Xiaomei;Shu Hu;Zhang Haifa;Shi Herong;
单 位：
Huang Ling%Li Xiaomei%Shu Hu%Zhang Haifa%Shi Herong
关键词：
hybrid grouper(E.fuscoguttatus ♀×E.lanceolatus ♂);;microecologics;;body composition;;intestinal digestive enzyme;;histological structure
摘 要：
The experiment aims at exploring the effects of two kinds of microecologics on body composition, intestinal digestive enzyme and histological structure of hybrid grouper(E. fuscoguttatus ♀×E.lanceolatus ♂),540 fishes with a body weight of(33.98±4.91) gram were randomly divided into sixgroups. A basal diet was supplemented with bacillus at 0.1%, 0.5% feed and with photosynthetic bacte-ria at 1%, 5% feed,These four kinds of feed were named Diet 1, Diet 2, Diet 3 and Diet 4 respectivelyas microecologics groups, Moreover emulsified fish oil group and control group would be set, and thetest period was 60 days. The results showed that:(1) Crude protein and crude fat decreased significant-ly as dietary probiotics(P<0.05), Crude ash were not statistically different(P>0.05).(2) The intestinaltrypsin and amylase activity decreased signifi-cantly as dietary probiotics(P<0.05), the lipaseactivity were not statistically different(P>0.05).(3) In the intestinal villus height of foregut, Com-pared with emulsified oil group, Diet 2 groupand Diet 3 group increased significantly(P<0.05),Diet 2 group increased significantly in the intesti-nal villus height of midgut(P<0.05), the muscle layer thickness and serous layer have no significance(P>0.05).(4) Probiotics can change the color ofthe fresh liver to some extent, reduce fat liver cell necrosis and cavitation.(5) Compared with controlgroup and emulsified oils group, The number of head kidney lymphocytes and melano-macrophage in-creased significantly, total head kidney cells and granulocyte have no significance(P>0.05). These re-sults indicated that dietary bacillus and photosynthetic bacteria could enhance the fish intestinal diges-tive enzymes and improve the intestines, liver and kidney structure of fish, and the then improve fish immunity.
