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当前位置: 首页 > 文章 > 黄花苜蓿不同种质材料的遗传多样性研究 中国草地学报 2013,35 (1) 61-66
Position: Home > Articles > Study on Genetic Diversity of Medicago falcata L. Germplasms Chinese Journal of Grassland 2013,35 (1) 61-66

黄花苜蓿不同种质材料的遗传多样性研究

作  者：
高素玲;郭丽;朱飞雪;杜建材;王照兰;胡卉芳
单  位：
河南农业职业学院;中国农业科学院草原研究所/农业部草原资源与生态重点开放实验室
关键词：
黄花苜蓿;种质材料;种子贮藏蛋白;遗传多样性
摘  要：
采用10%SDS-聚丙烯酰胺凝胶电泳技术,以单株取样的方法对黄花苜蓿4个品系和5份其他种质共9份供试材料的种子贮藏蛋白遗传多样性进行研究,并探讨了它们之间的遗传差异。结果表明:9份供试材料可溶性蛋白总条带数为69条,总多态带为56条,多态带百分率为81.16%;9份供试黄花苜蓿种质材料存在丰富的多样性,其遗传多样性顺序为8>6>1>5>4>3>2>9>7。5个相关种质含有比4个品系更加丰富的遗传多样性。9份供试材料的总基因多样性为0.2214,遗传分化系数为0.4929。4个品系的基因分化系数大于5个其他相关种质。基于Nei’s遗传距离,用UPGMA法进行聚类,9份供试材料可以分为4类,但聚类反映出的亲缘关系与群体取样的研究结果差异很大。
译  名：
Study on Genetic Diversity of Medicago falcata L. Germplasms
作  者：
GAO Su-ling1,GUO Li1,ZHU Fei-xue1,DU Jian-cai2,WANG Zhao-lan2,HU Hui-fang2(1.Henan Vocational College of Agriculture,Zhengzhou 451450,China;2.Grassland Research Institute,Chinese Academy of Agricultural Science/Key Laboratory of Grassland Resources and Ecology, Ministry of Agriculture,Hohhot 010010,China)
关键词：
Medicago falcata L.;Genetic resources;Seed storage proteins;Genetic diversity
摘  要：
Taking four strains(1,2,3,4) and five germplasms(5,6,7,8,9)of Medicago falcata L.as materials,using 10%gel and SDS-PAGE electrophoresis,the seed storage proteins of individuals were studied,and the genetic diversity and their inter-relationship were analyzed.The results showed that the total dissoluble protein bands of the nine materials were 69,there were 56 polymorphism bands,the ratio of polymorphism bands was 81.16%,there were plenty genetic diversity at protein level in the nine germplasms of Medicago falcata L.The diversity order were: 8>6>1> 5> 4> 3> 2> 9> 7.Total of five germplasms had higher genetic diversity than four strains.The total Nei's gene diversity index was 0.2214,and the total genetic differentiation coefficient was 0.4929.Genetic differentiation coefficient of the four strains was higher than the five germplasms.Based on the Nei's genetic distance and using UPGMA method to cluster,the nine genetic resources were clustered into four groups,but the genetic relationship was different from the result obtained with mixed seeds samples.

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