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Position: Home > Articles > Identification of Chromosome Ploidy of Vaccinium in the Northeast of China by Flow Cytometry Molecular Plant Breeding 2019 (4) 1257-1263

利用流式细胞术鉴定东北地区主要越桔属植物染色体倍性

作  者:
闫东玲;赵丽娜;王贺新;徐国辉;苏上;张明军;魏炳康;刘国玲
单  位:
大连大学现代农业研究院;大连大学生命科学与技术学院;大连森茂现代农业有限公司;中国林业科学研究院林业研究所
关键词:
越桔属;染色体倍性;流式细胞技术;笃斯越桔;朝鲜越桔
摘  要:
越桔属(Vaccinium)植物因其富含花青苷等生物活性成分而备受关注,中国东北地区越桔属植物资源丰富,但不同种类以及不同生态型的越桔属植物染色体倍性尚不十分清楚。为明确其主要种类的染色体倍性,本研究利用流式细胞技术对东北地区6种主要越桔属植物的染色体倍性进行了鉴定,结果表明,笃斯越桔(V. uliginosum L.)为四倍体,高山笃斯越桔(V. uliginosum var. alpinum E. Busch)为二倍体,红豆越桔(V.vitis-idaea L.)为四倍体,朝鲜越桔(又名红果越桔, Vaccinium koreanum Nakai)为四倍体,大果蔓越桔(又名红莓苔子, V. macrocarpon Ait.)为六倍体,小果蔓越桔(又名小果红莓苔子, V. microcarpum (Turcz.ExRupr.) Schmalh.)为二倍体。该研究将为中国越桔属植物资源的遗传学评价以及种质资源开发利用等提供重要的科学依据。
译  名:
Identification of Chromosome Ploidy of Vaccinium in the Northeast of China by Flow Cytometry
作  者:
Yan Dongling;Z hao Lina;Wang Hexin;Xu Guohui;Su Shang;Zhang Mingjun;Wei Bingkang;Liu Guoling;Life Science and Technology College,Dalian University;Institute of Modern Agricultural Research,Dalian University;Dalian Senmao Modern Agriculture Co.Ltd.;Institute of Forestry,Chinese Academy of Forestry;
关键词:
Vaccinium;;Chromosome ploidy;;Flow cytometry;;V.uliginosum L.;;Vaccinium koreanum Nakai
摘  要:
Vaccinium has attracted much attention because of its rich bioactive constituents such as anthocyanin.The resources of Vaccinium are very rich in Northeast of China. However, chromosome ploidy of different types and different ecotypes of Vaccinium is not very clear. In order to clarify the chromosome ploidy of Vaccinium major species, the chromosome ploidy of six main Vaccinium in Northeast of China were identified by Flow Cytometry in this study. The results showed that V. uliginosum L. was tetraploid, V. uliginosum var. alpinum E.Busch was diploid, V. vitis-idaea L. and Vaccinium koreanum Nakai were tetraploid. The large fruit cranberry(V. macrocarpon Ait.) was hexaploid, and the small fruit cranberry(V. microcarpum(Turcz.ExRupr.) Schmalh.)was diploid. The research would provide scientific basis for the genetic evaluation, exploitation and utilization of Vaccinium germplasm resources in China.

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