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当前位置: 首页 > 文章 > 航天诱变菌株黑曲霉ZM-8发酵玉米秸秆产β-葡萄糖苷酶的条件优化及其酶学特性 中国饲料 2012 (10) 14-17
Position: Home > Articles > Optium condition of β-glycosidase produced by the strain Aspergillus niger ZM-8 screened by space mutation with corn stalk and bran as materials China Feed 2012 (10) 14-17

航天诱变菌株黑曲霉ZM-8发酵玉米秸秆产β-葡萄糖苷酶的条件优化及其酶学特性

作  者：
马旭光;张宗舟;柳芸;赵国婵;霍建泰
单  位：
甘肃省航天育种工程技术研究中心;天水师范学院生命科学与化学学院
关键词：
航天诱变;黑曲霉ZM-8;β-葡萄糖苷酶;发酵条件;酶学特性
摘  要：
黑曲霉ZM-8是一株经航天诱变筛选出的高产纤维素酶菌株。以玉米秸秆和麸皮为原料,利用固态发酵法研究了发酵时间、发酵温度和初始pH对该菌株产β-葡萄糖苷酶的影响,采用正交试验对各因素进行了优化,并对酶学特性进行了初步研究。结果表明,发酵时间和发酵温度对酶活影响均达到极显著水平(F=14.994>F0.01=5.85;F=10.872),初始pH(F=5.843)对其影响达到显著水平;当初始pH为5.5、培养温度为35℃、培养时间为72 h时,β-葡萄糖苷酶的酶活达到了58.95 U/mL。该酶最适温度为55℃,保温2 h后仍具有95%以上的酶活力;最适pH为5.5,pH在3.0～6.0时,酶活力稳定性良好。
译  名：
Optium condition of β-glycosidase produced by the strain Aspergillus niger ZM-8 screened by space mutation with corn stalk and bran as materials
作  者：
Ma Xuguang,Zhang Zongzhou,Liu Yun,et al.
关键词：
space mutation;Aspergillus niger ZM-8;β-glycosidase;fermentation condition;enzymatic characterization
摘  要：
The strain Aspergillus niger ZM-8 with high cellulase activity was screened by space mutation with the corn stalk and bran as raw materials,the effects of fermentation time,fermentation temperature and initial pH on the β-glycosidase activity were studied by the method of solid-state fermentation and these factors were optimized by the orthogonal experiment,and the enzymatic characteristics were determined in this paper.The results showed that:the effects of fermentation time(F=14.994)and fermentation temperature(F=10.872) on the β-glycosidase activity were extremely significant,the effect of initial pH was significantly(F=5.843>F0.05=3.49).The β-glycosidase activity reached 58.95 U/mL under the conditions of initial pH was 5.5,fermentation temperature was 35 ℃ and fermentation time was 72 h.The β-glycosidase optimun temperature was 55 ℃ and the residual enzymatic activity was more than 95 % after being kept for 2 h.Its optimun pH was 5.5 and the enzyme activity was stable from pH 3.0 to 6.0.

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