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Position: Home > Articles > Polyploid Induction in Pear in vitro Treatment with Gamma-rays Acta Horticulturae Sinica 2009,36 (2) 257-260

γ-射线照射梨试管苗诱导产生多倍体变异

作  者:
孙清荣;孙洪雁;祝恩元;李林光
单  位:
山东省果树研究所;山东省科学技术厅
关键词:
梨;试管苗;γ-射线;多倍体
摘  要:
用γ-射线照射梨试管苗,照射后对其顶芽、侧芽和叶片3种外植体分别接种,经过继代和筛选,获得了形态变异明显的变异无性系,其变异表现为茎变粗,节间变短,叶长/叶宽比变小,叶柄变短。经染色体计数鉴定,这些变异中既有三倍体也有四倍体。多倍体的气孔长度及比叶重较对照二倍体显著增大。
译  名:
Polyploid Induction in Pear in vitro Treatment with Gamma-rays
作  者:
SUN Qing-rong1,SUN Hong-yan1,ZHU En-yuan2,and LI Lin-guang1(1Shandong Institute of Pomology,the State Agriculture Ministry Experiment Station of Temperate Fruits Germplasm Key Outdoor Observation in Tai'an,Shandong Fruits Biotechnology Breeding Key Laboratory,Tai'an,Shandong 271000,China;2Department of Science and Technology of Shandong Province,Jinan 250014,China)
关键词:
pear;in vitro shoots;gamma-ray;polyploid
摘  要:
In vitro shoots of pear cultivar'Fertility'(Pyrus communis L.) were irradiated with gamma-rays.Three different explants of shoot tip,lateral bud and young leaf from irradiated shoots were cultured separately.Shoots from 3 different explants were subcultured and selected.Morphological mutants with thicker stem,shorter internode,smaller leaf index and shorter petiole were obtained.Chromosome counting confirmed that the mutants were triploids and tetraploids.The lengths of stomatal guard cells and specific leaf mass of polyploids were significantly increased than that of diploids.

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