单 位：
山西农业大学动物科技学院;山西医科大学实验动物中心
关键词：
中国地鼠;线粒体DNA;D-loop基因;进化分析
摘 要：
本试验根据已知相近物种动物基因序列设计引物,PCR扩增获得中国地鼠线粒体D-loop基因序列。采用DNAS-tar软件计算序列碱基组成,分析遗传变异情况。用MEGA 4.1软件计算物种间遗传距离,采用邻接法(NJ)、最小进化法(ME)构建系统进化树。结果表明,中国地鼠线粒体D-loop区序列全长为867bp,碱基A、T、C、G的含量分别为29.53%、31.49%、26.07%、12.92%,中国地鼠与啮齿类动物有相似的碱基组成。系统进化树结果显示,中国地鼠与金黄地鼠亲缘关系较近,与大鼠的关系最远,与传统的分类相一致。所获得的中国地鼠D-loop基因可作为种间遗传变异研究的标记,研究结果为进一步研究中国地鼠的群体遗传结构奠定了基础。
译 名：
Cloning and Sequence Analysis of Mitochondrial D-loop Gene in Chinese Hamster
作 者：
SONG Guo-hua1,CHEN Chao-yang1,PANG Wen-biao1,GAO Ji-ping1,YUE Wen-bin2(1.Laboratory Animal Center,Shanxi Medical University,Taiyuan 030001,China; 2.College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China)
关键词：
Chinese hamster;mitochondrial DNA;D-loop gene;phylogenetic relationship
摘 要：
The primer was designed according to the closest animal published partial sequences,the PCR products were sequenced and determined.Combined with the known D-loop region sequence of other rodents,nucleotide composition were analyzed with DNAStar and genetic distance was analyzed with MEGA 4.1.The phylogenetic tree was constructed by neighbor-joining methods(NJ) and minimum-evolution methods(ME).The D-loop sequence of Chinese hamster had 867 bp,the nucleotide composition were relatively T(31.49%),C(26.07%),A(29.53%),and G(12.92%).The different clustering methods all showed a similar result.Chinese hamsters had the closest relationship with golden hamsters,but had relatively large difference from mice and rats,basically consistent with the traditional taxonomic status.D-loop gene might be genetic marker among species.The results of this study might be of importance for studies on evolution,structure and function of the mitochondria in Chinese hamsters.
