Position: Home > Articles > Water-compatible Molecularly Imprinted Solid Phase Extraction-HPLC for Determination of Fluorouinolones Residues in Chicken
FOOD SCIENCE
2009,30
(8)
151-154
水兼容性分子烙印固相萃取-高效液相色谱法测定鸡肉中氟喹诺酮类药物残留
作 者：
孙汉文;刘广宇;乔凤霞
单 位：
河北大学化学与环境科学学院;河北大学化学与环境科学学院,河北省分析科学技术重点实验室
关键词：
分子烙印聚合物;固相萃取;高效液相色谱;氟喹诺酮;鸡肉
摘 要：
目的:建立分子烙印固相萃取-高效液相色谱测定鸡肉中氟喹诺酮类药物残留的新方法。方法:以甲基丙烯酸为功能单体,恩诺沙星为模板分子,在强极性溶剂甲醇-水体系中制备分子烙印聚合物,考察和评价分子烙印聚合物的特性。鸡肉样品经乙腈提取浓缩后过分子烙印固相萃取柱,乙腈-三氟乙酸(99:1,V/V)洗脱液由高效液相色谱分离和荧光法检测。结果:高亲合力的结合位点的解离常数为Kd1=7.19×10-5mol/L,最大表观结合位点数Qmax,1=110.19μmol/g;低亲和力结合位点的解离常数为Kd2=2.44×10-3mol/L,最大表观结合位点数Qmax,2=965.51μmol/g。氧氟沙星、诺氟沙星、环丙沙星和恩诺沙星等氟喹诺酮类药物的校准曲线在1.0～500μg/kg范围内呈良好的线性关系(r≥0.9991),检出限(S/N=3)为0.06～0.09μg/kg,平均回收率为75.4%～86.4%(n=3),相对标准偏差小于6%。结论:以水兼容性分子烙印固相萃取-高效液相色谱法可实现氟喹诺酮药物的有效分离和灵敏测定。
译 名：
Water-compatible Molecularly Imprinted Solid Phase Extraction-HPLC for Determination of Fluorouinolones Residues in Chicken
作 者：
SUN Han-wen,LIU Guang-yu,QIAO Feng-xia(Key Laboratory of Analytical Science and Technology of Hebei Province,College of Chemical and Environmental Sciences,Hebei University,Baoding 071002,China)
关键词：
molecularly imprinted polymers;solid-phase extraction;HPLC;fluoroquinolones;chicken
摘 要：
Objective:To establish a new method for the effective separation and sensitive detection of four fluorouinolones(ofloxacin,norfloxacin,ciprofloxacin and enrofloxacin) residues in chicken by using molecularly imprinted solid phase extraction-HPLC.Methods:Water-compatible molecularly imprinted polymers(MIPs) were synthesized in methanol-water systems with enrofloxacin as templates and methacryclic acid as functional monomers,and their characteristics were investigated and evaluated.The chicken samples were extracted with acetonitrile,purified by molecularly imprinted solid-phase extraction column with mixture of acetonitrile and trifluoracetic acid(99:1,V/V) as elution solution,and then the eluent was separated by HPLC and detected by fluorescence method.Results:The MIPs have good adsorption specificity to six fluoroquinolones.For the higher affinity site,Kd1 is 7.19 × 10-5 mol/L,and Qmax,1 is 110.19 μmol/g;For the lower affinity site,Kd2 is 2.44 × 10-3 mol/L,and Qmax,2 965.51 μmol/g.Excellent linear relationships may be obtained in the range of 1.0 to 500 μg/kg(r ≥ 0.9991).The determination limits(S/N= 3) are in the range of 0.06 to 0.09 μg/kg.The mean recoveries range from 75.3% to 88.5% with relative standard deviations below 6%(n = 3).Conclusion:The effective separation and sensitive detection of the four fluorouinolones in chicken can be archived by using the water-compatible molecularly imprinted solid phase extraction-HPLC.
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