中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > RNAi介导的棉铃虫细胞色素P450酶系几种组分基因沉默对高效氯氰菊酯毒力的影响(英文) 植物保护学报 2013,40 (4) 355-362
Position: Home > Articles > Effects of RNAi-mediated silencing of several components of cytochrome P450s on beta-cypermethrin toxicity against Helicoverpa armigera Journal of Plant Protection 2013,40 (4) 355-362

RNAi介导的棉铃虫细胞色素P450酶系几种组分基因沉默对高效氯氰菊酯毒力的影响(英文)

作  者：
唐涛;赵春青;冯晓云;刘雪源;邱立红
单  位：
湖南省农业科学院植物保护研究所;中国农业大学理学院
关键词：
棉铃虫;细胞色素P450酶系;RNA干扰;抗药性;实时定量PCR
摘  要：
为进一步明确细胞色素P450 CYP6B7、细胞色素b5(cytochrome b5,Cyt-b5)和细胞色素P450还原酶(cytochrome P450 reductase,CPR)在棉铃虫Helicoverpa armigera(Hübner)对拟除虫菊酯类药剂抗性中的作用,通过RNA干扰技术沉默了抗氰戊菊酯棉铃虫中3种组分的基因,并测定基因沉默后高效氯氰菊酯对棉铃虫的毒力变化。结果显示,经单个CYP6B7或CYP6B7与CPR、Cyt-b5基因的双链RNA注射处理后12~48 h,棉铃虫幼虫死亡率在19.4%~92.5%之间,明显高于空白对照处理的10.47%~62.87%;高效氯氰菊酯对基因沉默后幼虫的LD50值随着时间的延长而逐渐下降,12~48 h的LD50值在0.97~2.97μg/头之间,而空白对照处理为2.27~3.57μg/头。研究表明,细胞色素P450 CYP6B7,或CYP6B7与CPR、Cyt-b5基因的沉默,可提高高效氯氰菊酯对抗性棉铃虫的毒力,进一步证明了CYP6B7、CPR和Cyt-b5在棉铃虫对菊酯类药剂抗性中起着重要作用。
译  名：
Effects of RNAi-mediated silencing of several components of cytochrome P450s on beta-cypermethrin toxicity against Helicoverpa armigera
作  者：
Tang Tao;Zhao Chunqing;Feng Xiaoyun;Liu Xueyuan;Qiu Lihong;Institute of Plant Protection,Hunan Academy of Agricultural Sciences;College of Sciences,China Agricultural University;
关键词：
Helicoverpa armigera;;cytochrome P450s;;RNA interference;;resistance;;real-time quantitative PCR
摘  要：
To further clarify the role of cytochrome P450 CYP6B7,cytochrome b 5(Cyt-b 5) and cytochrome P450 reductase(CPR) in a Handan fenvalerate-resistant strain of Helicoverpa armigera(HDFR),the genes of the three components were silenced with RNA interference(RNAi) strategies,and the toxicity of beta-cypermethrin against H.armigera was assessed.The results showed that the mortalities of the double-stranded RNA(dsRNA)-injected larvae of H.armigera were 19.4%-92.5%,which were significantly higher than those of the control(10.47%-62.87%).The LD 50 values of beta-cypermethrin to the dsRNA-injected larvae were 0.97-2.97 μg / larva,while those of the control were 2.27 3.57 μg / larva during a 12-48 h period,and the LD 50 values decreased as the exposure time to betacypermethrin increased.The present study indicated that silencing of CYP6B7 alone or together with CPR and / or Cyt-b 5 genes increased the toxicity of beta-cypermethrin against the larvae of fenvalerate-resistant strain of H.armigera,further suggesting that CYP6B7,CPR and Cyt-b 5 played crucial roles in the resistance of H.armigera to pyrethroids.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46