摘 要：
用PCR方法从谷氨酸棒杆菌突变株ZL9601基因组中扩增获得了天冬氨酸激酶(AK)编码基因,并对该基因编码区(1263bp)进行了序列分析。结果表明:本文扩增的AK基因编码与文献报道的序列基本一致,通过与不同作者克隆的AK基因序列进行比较,同源性最高达99 7%,最低达97 2%。这些碱基的差异均表现为碱基取代,多数碱基取代未引起编码氨基酸的改变,仅有少数碱基取代导致编码氨基酸的变化。由此可见,谷氨酸棒杆菌AK基因的碱基序列存在着多态性。
译 名：
Cloning and sequence analysis of the aspartokinase (AK) encoding gene
作 者：
LI Xiu-jin,ZHONG Fei(Department of Biotechnology of College Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China)
关键词：
AK gene; cloning; sequence analysis; Croynebacterium glutamicum
摘 要：
Aspartokinase (AK) gene was cloned with PCR from Croynebacterium glutamicum mutant strain Zl-9601 and compared with previously reported polymorphic AK gene sequences. The cloned AK gene sequence in this study is highly homologous with other AK gene sequences, ranging from 97.2 % to 99.7%. Most of the substituted nucleotide bases did not cause any changes to the amino acid sequence of the protein, which comes to the conclusion that polymorphism exists in the AK gene.
