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Position: Home > Articles > New Technologies of Nucleic Acid Amplification Progress in Veterinary Medicine 2010,31 (3) 103-106

核酸扩增新技术

作  者:
鲁雪;李赟;黄倢
单  位:
中国水产科学研究院黄海水产研究所;中国海洋大学水产学院
关键词:
LAMP技术;HDA技术;固相PCR技术;SELEX技术;SOLEXA技术
摘  要:
核酸扩增技术广泛应用于生命科学及其相关的各个领域,对生命科学的发展发挥着重要的作用。论文对核酸扩增的一些新技术进行综述,包括环介导等温扩增(LAMP)技术,赖解旋酶恒温基因扩增(HDA)技术,固相PCR技术,指数富集配体的系统进化(SELEX)技术,SOLEXA高通量测序技术。这些新技术具有重要的应用价值,随着技术的不断完善,将会有广阔的应用前景。
译  名:
New Technologies of Nucleic Acid Amplification
作  者:
LU Xue1,2,LI Yun1,HUANG Jie2(1.College of Fisheries,Ocean University of China,Qingdao,Shandong,266003,China;2.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong,266071,China)
关键词:
LAMP;HDA;solid phase PCR;SELEX;SOLEXA
摘  要:
At present,nucleic acid amplification technologies have been widely used in life science and its correlative fields,and play an important role in the development of life science.Some of the new nucleic acid amplification technologies,including LAMP(loop-mediated isothermal amplification),HDA(helicase-dependent isothermal DNA amplification),solid phase PCR,SELEX(systematic evolution of ligands by exponential enrichment)and SOLEXA high throughput sequencing,were summarized in this review.These new technologies have significant application values and show broad future prospects with their perfection.

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