Position: Home > Articles > 适用于猪场沼液处理的好氧反硝化菌筛选及其脱氮特性评价
Chinese Journal of Animal Science
2019
(6)
113-120
适用于猪场沼液处理的好氧反硝化菌筛选及其脱氮特性评价
作 者：
孙宏;吴逸飞;张恒;沈琦;姚晓红;王新;汤江武;葛向阳
单 位：
浙江省农业科学院植物保护与微生物研究所
关键词：
氧反硝化;猪场沼液;脱氮性能;假单胞菌
摘 要：
试验旨在筛选适合于猪场沼液处理的好氧反硝化菌.从活性污泥中分离获得16株好氧反硝化菌,其中菌株ZH-14的总氮(TN)和硝酸盐氮的去除效果最好,分别达到50.73%和99.99%.该菌株经过平板形态观察、功能基因和16S rDNA基因分析,鉴定为施氏假单胞菌(Pseudomonas stutzeri).结果表明:菌株ZH-14在硝酸钾为唯一氮源培养基中生长时,48 h后硝酸盐氮与TN的降解率分别为100%和41.76%;以亚硝酸钠为唯一氮源培养时,亚硝酸盐氮降解率为99.24%;以氨氮为唯一氮源培养时,氨氮的降解率达94.1%;使用菌株ZH-14处理畜禽沼液,当其接种终浓度为10~7CFU/mL时,经过48h处理,氨氮、硝酸盐氮和化学需氧量的去除率分别为54.4%、97.7%和77.9%.综上表明,菌株ZH-14具有较强的反硝化和处理猪场沼液的能力,具有良好的应用开发前景.
作 者：
SUN Hong;WU Yi-fei;ZHANG Heng;SHEN Qi;YAO Xiao-hong;WANG Xin;TANG Jiang-wu;GE Xiang-yang;Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University;
单 位：
SUN Hong%WU Yi-fei%ZHANG Heng%SHEN Qi%YAO Xiao-hong%WANG Xin%TANG Jiang-wu%GE Xiang-yang%Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences%State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University
关键词：
Aerobic denitrification;;Piggery biogas liquid;;Denitrification characteristics;;Pseudomonas sp
摘 要：
The aim of the present study is to screen aerobic denitrifying bacteria for the treatment of piggery biogas liquid.Sixteen aerobic denitrifying strains were selected from actived sludge. Among them, stain ZH-14 had the best removal rate of total nitrogen(TN) and nitrate nitrogen, reaching 50.73% and 99.99%, respectively. The strain was identified as Pseudomonas stutzeri by plate morphology, functional genes and 16 S rDNA sequence analysis. The results showed that the maximum degradation rates of nitrate nitrogen and TN reached 100% and 41.76%, respectively, when it grew in the medium with potassium nitrate as the sole nitrogen source after 48 h's cultivation. When sodium nitrite was used as the sole nitrogen source for culturing strain ZH-14, the maximum degradation rate of nitrite nitrogen was 99.24% within 24 h. When strain ZH-14 was cultured with ammonia nitrogen as the sole nitrogen source, the degradation rate of ammonia nitrogen was 94.1% after 24 h. For the treatment of piggery biogas liquid, the removal rate of ammonia nitrogen, nitrate nitrogen and chemical oxygen demand were 54.4%, 97.7%, and 77.9%, respectively, after 48-h treatment of strain ZH-14 at a final inoculation concentration of 10`7 CFU/mL. The result indicates that strain ZH-14 has a good application prospect in biological denitrification and the treatment of piggery biogas liquid.
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