当前位置: 首页 > 文章 > 赤拟谷盗和杂拟谷盗体内Wolbachia的感染状况和种间传播 江苏农业学报 2015,31 (2) 272-278
Position: Home > Articles > Infection and interspecific transmission of Wolbachia in Tribolium castaneum and T. confusum Jiangsu Journal of Agricultural Sciences 2015,31 (2) 272-278

赤拟谷盗和杂拟谷盗体内Wolbachia的感染状况和种间传播

作  者:
程超;沈加飞;明庆磊
单  位:
江苏师范大学生命科学学院
关键词:
赤拟谷盗;杂拟谷盗;沃尔巴克氏体;水平传播;垂直传播
摘  要:
为明确沃尔巴克氏体(Wolbachia)在赤拟谷盗和杂拟谷盗体内的感染状况及种间水平和垂直传播情况,首先通过PCR扩增分离细胞分裂蛋白基因(fts Z)目的片段方法检测这2个近缘种拟谷盗体内是否存在Wolbachia,并将同性别或异性别的赤拟谷盗和杂拟谷盗混合饲养40 d,然后测定赤拟谷盗及其与杂拟谷盗的正反交杂种后代体内Wolbachia的感染状况。结果显示,杂拟谷盗体内存在Wolbachia,而赤拟谷盗体内不存在该菌;混合饲养后,赤拟谷盗及其与杂拟谷盗的正反杂交后代体内也均没有检测到Wolbachia的存在,未发现Wolbachia在杂拟谷盗和赤拟谷盗种间的水平和垂直传播。
译  名:
Infection and interspecific transmission of Wolbachia in Tribolium castaneum and T. confusum
作  者:
CHENG Chao;SHEN Jia-fei;MING Qing-lei;School of Life Sciences,Jiangsu Normal University;
关键词:
Tribolium castaneum;;T.confusum;;Wolbachia;;horizontal transmission;;vertical transmission
摘  要:
To define the infection and interspecifically horizontal and vertical transmission of Wolbachia in two closely related flour beetles Tribolium castaneum( Herbst) and T. confusum( Jac. du Val.),Wolbachia was detected in both beetle species by PCR amplification of fts Z target gene fragment. After same-sex or opposite-sex mixed feeding,the infection of Wolbachia in the red flour beetles T. castaneum and the reciprocal hybrids of T. castaneum and T. confusum was examined. The wolbachia infected confused flour beetles T. confusum exclusively. The red flour beetles T. castaneum and the reciprocal hybrids were Wolbachia-free. These suggests that interspecifically horizontal and vertical transmissions of Wolbachia did not occur in the two closely related flour beetles T. castaneum and T. confusum,and T. castaneum may have an internal mechanism inhibiting the reproduction and survival of Wolbachia.

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