The Colonization,Bacteriostasis and Purification Water of Marine Paenibacillus polymyxa Strain L_1-9 in a Japanese Shrimp Culture Pond

BAO Zeng-hai;MA Gui-zhen;WANG Shu-fang;FU Hong-run;GE Ping-hua;WU Shao-jie;Huaihai Institute of Technology;

marine Paenibacillus polymyxa;;bacteriostasis;;purification water

Marine Paenibacillus polymyxa strain L1-9 was inoculated(without inoculation as control)in Japanese shrimp culture ponds to investigate the colonization,bacteriostasis and purification water of the marine P.polymyxastrain L1-9in the Japanese shrimp culture ponds.After that,the number of L1-9,Vibrio,Bacillaceae and heterotrophic bacteria was detected regularly,and the concentrations of ammonia nitrogen,nitrate,nitrite,and phosphate were detected.During the culture,the number of Vibrio,Bacillaceae,and heterotrophic bacteria and the levels of ammonia nitrogen,nitrate,and nitrite were found to be significantly lower in the Japanese shrimp culture ponds inoculated with L1-9 strain than those in the control.However,there was significantly higher concentration of phosphate in the Japanese shrimp culture ponds inoculated with L1-9 strain than that in the control pond,especially the number of Bacillaceae and the ammonia nitrogen level,the Bacillaceae reaching 4.60×102 cfu/mL on the 5th d,significantly less than that in the control(1.80×103 cfu/mL)on the 7th d.The concentration of ammonia nitrogen was found to be 60.2μg/L,less than that in the control(76.7μg/L).It is concluded that L1-9strain has obviously bacteriostasis and purification water effect in aquaculture.