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Position: Home > Articles > Flow Injection Analysis of Nitrate and Nitrite in Vegetables FOOD SCIENCE 2009,30 (16) 272-274

流动注射法测定蔬菜中的硝酸盐和亚硝酸盐含量

作  者:
任乃林;李红
单  位:
韩山师范学院化学系
关键词:
硝酸盐;亚硝酸盐;流动注射;Zn-C柱还原;分光光度法
摘  要:
基于NO3-可通过Zn-C柱还原为NO2-,结合流动注射分析技术,建立了流动注射分光光度法测定蔬菜中硝酸盐和亚硝酸盐含量的新方法。样品匀浆后以超声波提取、活性炭脱色后,提取液一份直接用盐酸萘乙二胺比色法测定其中的亚硝酸盐,另一份经Zn-C柱将硝酸盐还原为亚硝酸盐后用相同方法测定,差减法计算硝酸盐含量。其中亚硝酸盐方法的线性范围为0.1~2.4μg/ml,检出限为0.01μg/ml,相对标准偏差介于0.03%~1.8%之间,样品测定频率为10样/h。
译  名:
Flow Injection Analysis of Nitrate and Nitrite in Vegetables
作  者:
REN Nai-lin,LI Hong(Department of Chemistry,Hanshan Normal University,Chaozhou 521041,China)
关键词:
nitrate;nitrite;flow injection analysis(FIA);Zn-C column reduction;spectrophotometry
摘  要:
Based on the reduction from nitrate to nitrite through a Zn-C powder mixture column,a new method for the determination of nitrate and nitrite in vegetables using flow injection analysis were proposed.Samples were homogenized and extracted,and the extract was decolorized and divided into two portions.The nitrite content in one portion was directly determined by hydrochloride naphthalene ethylenediamine colorimetry,and another was determined by the same method after nitrate was reduced to nitrite.The content of nitrate was calculated by subtraction method.The linear range of nitrite was 0.12.4μg/ml,the detection limit 0.01 μg/ml,the range of relative standard deviation from 0.03% to1.8%,and the sample determination frequency 10 samples/h.

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