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当前位置: 首页 > 文章 > 花花柴脂肪酸合成相关基因KcFabZ的克隆及分析 塔里木大学学报 2019 (2) 23-29
Position: Home > Articles > 花花柴脂肪酸合成相关基因KcFabZ的克隆及分析 Journal of Tarim University 2019 (2) 23-29

花花柴脂肪酸合成相关基因KcFabZ的克隆及分析

作  者：
王磊;王彦芹
单  位：
塔里木大学生命科学学院
关键词：
高温;花花柴;脂肪酸;FabZ;qRT-PCR
摘  要：
脂肪酸是生物体内的重要能源物质.为深入分析花花柴脂肪酸合成相关基因FabZ(β羟酰基ACP脱水酶)的结构与功能,通过PCR克隆获得该基因核苷酸序列,命名为KcFabZ.对该基因的编码蛋白进行了生物信息学分析,并利用荧光定量PCR分析其在高温处理下的表达模式.结果显示:KcFabZ基因全长为669 bp,编码232个氨基酸.定位在叶绿体中;系统发育分析结果显示KcFabZ与菊科莴苣中的该基因同源性达到89%;根据NCBI中保守结构域预测,KcFabZ为hot_dog超家族功能基因,具有典型的α+β'hot_dog'折叠;荧光定量PCR结果显示,KcFabZ表现在高温处理下随着处理时间的延长表达量先升高后降低,高温处理8 h时表达量达到最大值,为对照的19. 54倍,以上研究结果表明,KcFabZ可能与花花柴对高温胁迫的响应相关.
作  者：
Wang Lei;Wang Yanqin;College of Life Sciences,Tarim University;
单  位：
Wang Lei%Wang Yanqin%College of Life Sciences,Tarim University
关键词：
high temperature;;Karelinia capsia;;fatty acid;;FabZ;;qRT-PCR
摘  要：
Fatty acids are important energy substances in living organisms. In order to deeply analyze the structure and function of fatty acid synthesis related gene FabZ of Karelinia capsia, The nucleotide sequence of the gene was obtained by PCR cloning and named for KcFabZ. The bioinformatics analysis of the encoded protein of the gene was carried out, and its expression pattern under high temperature treatment was explored by real-time PCR. Results showed that the length of KcFabZ gene was 669 bp and coded 232 amino acids, which was localized in chloroplasts. The phylogenetic results showed that Kc FabZ shared 89% homology with Lactuca sativa Linn. According to the conserved domain prediction in NCBI, KcFabZ is a hot_dog superfamily functional gene, with typical α+β'hot_dog'folding. The results of real-time PCR showed that the expression of KcFabZ increased first and then decreased with the treatment time under high temperature treatment. The expression reached the maximum at 8 h after high temperature treatment,which was 19.54 times of the control. The above results indicate that KcFabZ may be related to The response of Karelinia capsia to high temperature stress is related.

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