Position: Home > Articles > Identification and Virulent Test of Rabies Virus Isolates and Study on their Detection with Nucleotide Probe
Journal of Jilin Agricultural University
1997
(1)
3-9
RV野毒株的鉴定、毒力实验及其核酸探针的研究
作 者：
钱爱东;侯世宽;何昭阳;刘清河;殷震
单 位：
吉林农业大学动物科学系
关键词：
狂犬病病毒,鉴定,核酸探针
摘 要：
对从我国不同地区、不同动物分离的３个疑似狂犬病毒分离物８２０２、ＢＲＶ、ＭＲＶ和无毒疫苗株ＳＲＶ９进行了ＥＬＩＳＡ和核酸探针的鉴定。同时，对它们的致病性和毒力进行了测定。结果表明，３个分离物均为狂犬病病毒（ＲＶ），其毒力以鼠源ＭＲＶ最强，牛源ＢＲＶ最弱，同地不同动物分离的ＢＲＶ和ＭＲＶ毒力差异很大。疫苗株ＳＲＶ９对１２ｇ小鼠无致病性。研制的狂犬病毒地高辛标记核酸探针敏感性高、特异性强，适用于对狂犬病病料进行诊断和检查
译 名：
Identification and Virulent Test of Rabies Virus Isolates and Study on their Detection with Nucleotide Probe
作 者：
Qian Aidong et al. (Dept.of Animal Science, Jilin Agricultural University, Changchun 130118)
关键词：
rabies virus; identification; nucleotide probe
摘 要：
Three isolates(8202, MRV, BRV) from the brain tissue of deer, calf, mouse that died of suspectable rabies were identified as rabies virus by ELISA and nucleotide probe. The results showed that LD 50 of MRV strain is the highest (6.1) , LD 50 of BRV strain is the lowest (4.1), 8202 strain is 5.1. Avirulent strain SRV 9 had no pathogenicity to mice. Virulence of BRV and MRV from the same area is different obviously. The nucleotide probe labeled with digoxigenin dUTP for G gene of rabies virus is senstive and specific. The probe exhibited potential of detecting nucleic acid of rabies virus from infected brain tissues.
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