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Position: Home > Articles > Separation and purification of SEB Science and Technology of Food Industry 2009 (2) 136-138

金黄色葡萄球菌肠毒素B的分离纯化

作  者:
朱俊友;李玉锋
单  位:
安阳工学院生物与食品工程学院;西华大学生物工程学院
关键词:
金黄色葡萄球菌;肠毒素B;分离纯化
摘  要:
为了分离纯化金黄色葡萄球菌肠毒素B(SEB),在离心、浓缩后,将浓缩液依次经羧甲基阳离子交换纤维素CM-32和葡聚糖凝胶G-75两步柱层析,再经SDS-聚丙烯酰胺凝胶电泳分析,获得了电泳纯的金黄色葡萄球菌肠毒素B。实验证明,该简化的二步柱层析分离得到的SEB纯度高,方法简便易行,一般实验室均可采用。
译  名:
Separation and purification of SEB
作  者:
ZHU Jun-you1,LI Yu-feng2(1. College of Biology and Food Engineering,Anyang Institute of Technology,Anyang 455000,China;2.College of Bioengineering,Xihua Univercity,Chengdu 610039,China)
关键词:
Staphylococcus aureus;staphylococcal enterotoxin B;separation and purification
摘  要:
To separation and purification of SEB,positive ion-exchange carboxyl methyl cellulose CM-32 and Sephadex G-75 were used by centrifugal and concentrated methods.At last,by SDS-PAGE electrophoresis analysis,pure SEB could be gained.It proved that only two steps could obtain high purity SEB,the method was simple and ecumenic,and it could be used in the laboratory.

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