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当前位置: 首页 > 文章 > 控制溶解氧浓度对里氏木霉产纤维素酶的促进作用 南京林业大学学报(自然科学版) 2008,32 (3) 57-60
Position: Home > Articles > Effects of controlling dissolved oxygen tension on cellulase production by Trichoderma reesei Journal of Nanjing Forestry University(Natural Sciences Edition) 2008,32 (3) 57-60

控制溶解氧浓度对里氏木霉产纤维素酶的促进作用

作  者：
杭志喜;饶庆隆;余世袁
单  位：
南京林业大学化学工程学院
关键词：
里氏木霉;纤维素酶;溶解氧浓度;CO_2含量;滤纸酶活
摘  要：
通过调节通风量控制溶解氧浓度来研究其对里氏木霉产纤维素酶的促进作用。结果表明:当通风量固定为25L/(h·L)时,反应22~40h期间溶解氧浓度过低,0~15h和45h以后溶解氧浓度偏高,45~80h期间气体中CO2含量在0.8%以上,不利于菌丝的生长。调节通风量控制溶解氧浓度,可有效地提高里氏木霉合成纤维素酶的能力,并显著地缩短产酶时间。当溶解氧为20%~30%时,气体中CO2含量降到0.4%左右,菌丝生长代谢快,滤纸酶活从固定通风量时的2.75U/mL增加到3.54U/mL,提高28.7%,产酶酶活最高的时间也从84h缩短到72h。
译  名：
Effects of controlling dissolved oxygen tension on cellulase production by Trichoderma reesei
作  者：
HANG Zhi-xi1,2,RAO Qing-long1,YU Shi-yuan1(1.College of Chemical Engineering Nanjing Forestry University,Nanjing 210037,China;2.Department of Biochemical Engineering Anhui University of Technology and Science,Wuhu 241000,China)
关键词：
Trichoderma reesei;Cellulase;Dissolved oxygen tension(DOT);Content of CO_2;Filter paper activity
摘  要：
The effects of controlling dissolved oxygen tension(DOT) on cellulase production by Trichoderma reesei were studied with 10 g/L pulp.The moore content of carbon dioxide and oxygen in off-gass and cellulase activity were mensurated.The results showed that at aeration rate of 25 L/(h·L),the DOT was too low for 22-40 h,but it was high for 0-15 h and 45 h to end.The moore content of carbon dioxide in the gas was above 0.8% for 45-80 h,and it went against the growth of mycelium.When the aeration was adjusted to control DOT,cellulase activity was effectively increased and the time of cellulase production was evidently shorten.At DOT of 20%-30%,the content of carbon dioxide in the gas was dropped to about 0.4 %,the growth and metabolize of mycelium was better and filter paper activity(FPA) reached 3.54 U/mL.It was 28.7% higher than 2.75 U/mL of the unchanged one.It was found that the optimum time for harvest of cellulase was shortened from 84 h to 72 h.

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