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Hubei Agricultural Sciences
2015,54
(18)
4570-4572+4577
茶叶生产废料中表没食子儿茶素没食子酸酯的纯化工艺
作 者：
许敏;黄思勇;周光龙;胡平;彭经寿;金显平
单 位：
恩施职业技术学院
关键词：
茶叶生产废料;表没食子儿茶素没食子酸酯(EGCG);纯化
摘 要：
分析了茶叶生产废料中表没食子儿茶素没食子酸酯(EGCG)的纯化工艺,并采用大孔树脂对其进行分离纯化。结果表明,运用静态吸附与动态吸附相结合的方法分析得到,适用于茶叶生产废料中EGCG分离纯化用的大孔吸附树脂为D101。其最佳分离纯化条件为:将20.0 mg/m L上柱液以1.5 BV/h上柱4.5 BV,使用20%乙醇以0.5 BV/h洗脱4.0 BV。
译 名：
Purification Process of Epigallocatechin-3-gallate in Tea Production Waste
作 者：
XU Min;HUANG Si-yong;ZHOU Guang-long;HU Ping;PENG Jing-shou;JIN Xian-ping;Enshi Technical College;
关键词：
tea production waste;;epigallocatechin-3-gallate;;green extraction process
摘 要：
An orthogonal test was used to optimize the extraction technology of EGCG from tea production waste,and to separate and purify the Epigallocatechin-3-gallate by macroporous resin.The results showed the optimal conditions for separation and purification were: D101 macroporous resin, the concentration of sample was 20.0 mg/m L, the volume of sample was 4.5times of column volume, flow rate was 1.5 BV / h 20% ethanol for elution, elution velocity was 0.5 BV / h while elution volume was 4.0 BV.
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