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当前位置: 首页 > 文章 > 星豹蛛细胞色素CYP3001U15基因克隆与生物信息学分析 山西农业科学 2020 (6) 842-846
Position: Home > Articles > Cloning and Bioinformatics Analysis of CYP3001U15 Gene in Pardosa astrigera(Araneae:Lycosidae) Journal of Shanxi Agricultural Sciences 2020 (6) 842-846

星豹蛛细胞色素CYP3001U15基因克隆与生物信息学分析

作  者：
任彦鸿;王雅丽;赵瑞;田国强;燕晶晶;李锐
单  位：
关键词：
星豹蛛;细胞色素P450;基因克隆;生物信息学分析
摘  要：
星豹蛛是农业害虫的重要捕食性天敌。通过转录组测序鉴定出66条星豹蛛细胞色素P450基因,进一步筛选分析出5条基因,预测其与外源物质代谢有关。为进一步研究星豹蛛细胞色素P450功能和代谢机制,选取其中一条基因进行生物信息学研究。试验通过RT-PCR扩增技术,将星豹蛛总RNA反转录cDNA,后以此为模板进行PCR扩增,回收纯化产物完成单克隆并测序,获取该基因序列信息,并对其进行生物信息学分析。结果发现,克隆到CYP3001U15基因ORF序列,该基因开放阅读框为1 074 bp,编码357个氨基酸,分子质量为42 ku,等电点为5.91,原子总数为5 808,分子式为C_(1877)H_(2896)N_(494)O_(531)S_(10);正电荷残基47个,占氨基酸总数的13.2%,负电荷残基52个,占氨基酸总数的14.6%;蛋白的二级结构预测结果显示,α螺旋占47.62%,β折叠占8.96%,β转角占4.76%,无规则卷曲占38.66%;亚细胞定位预测结果显示,其定位在细胞内质网上;蛋白信号肽预测结果显示,该蛋白不存在信号肽;跨膜结构预测分析结果显示,其不存在跨膜结构。研究结果可就星豹蛛细胞色素P450基因对杀虫剂等外源物代谢的研究提供理论基础。
译  名：
Cloning and Bioinformatics Analysis of CYP3001U15 Gene in Pardosa astrigera(Araneae:Lycosidae)
作  者：
REN Yanhong;WANG Yali;ZHAO Rui;TIAN Guoqiang;YAN Jingjing;LI Rui;College of Agronomy,Shanxi Agricultural University;
关键词：
Pardosa astrigera;;cytochrome P450;;gene cloning;;bioinformatics analysis
摘  要：
Pardosa astrigera is an important natural enemy of agricultural pests, by transcriptome sequencing identified 66 cytochrome P450 genes, further screening analysis article 5 genes and predict its related to metabolism of exogenous substances,to study more function and metabolic mechanism of cytochrome P450 enzyme system of Pardosa astrigera, select one of the genes to research and provide theoretical support for the subsequent studies on resistance. By using RT-PCR technology, the total RNA was amplified by reverse transcription PCR, cloning and sequencing were performed with the amplified products recovered from cutting gum,and bioinformatics analyses of CYP3001U15 gene. The results showed that CYP3001U15 gene ORF sequence was cloned. The open reading frame of the gene was 1 074 bp, encoding 357 amino acids. Its molecular quality was 42 ku, isoelectric point was 5.91, the total number of atoms was 5 808, and the molecular formula was C_(1877)H_(2896)N_(494)O_(531)S_(10). Positively charged residues were 47, accounting for 13.2% of total amino acids, negatively charged residues were 52, accounting for 14.6% of the total amino acids. On its secondary structure, the alpha helix was 47.62%, extend strand was 8.96%, beta turn was 4.76%, random coil was 38.66%. Subcellular localization prediction showed on endoplasmic reticulum. The results of signal peptide prediction showed that there was no signal peptide in the protein. The prediction of transmembrane region structure of the protein showed that there was no transmembrane structure. This study result will provide theoretical foundation for the future study on cytochrome P450 gene of Pardosa astrigera.

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