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当前位置: 首页 > 文章 > 冬小麦‘东农冬麦1号’成熟胚组织培养及再生体系建立 东北农业大学学报 2015 (11) 8-15
Position: Home > Articles > Establishment of tissue culture and regeneration system from mature embryos of winter wheat Dongnongdongmai1 Journal of Northeast Agricultural University 2015 (11) 8-15

冬小麦‘东农冬麦1号’成熟胚组织培养及再生体系建立

作  者：
王军虹;郑成成;于晶;卢秋巍;吴冰;朱会杰;冯明芳;梅琳;李维娜;褚丽阳
单  位：
东北农业大学生命科学学院
关键词：
冬小麦;东农冬麦1号;成熟胚;组织培养;再生体系
摘  要：
‘东农冬麦1号’是目前黑龙江省高寒地区首个可越冬冬小麦品种,建立高效稳定组织培养再生体系,对品种改良和功能基因组学研究具有重要意义。研究以‘东农冬麦1号’成熟胚为外植体,探讨培养基类型、接种方式、添加物组合浓度、2,4-D、6-BA和KT浓度等因素对愈伤组织诱导、分化及植株再生影响。结果表明,不同培养基类型对冬小麦成熟胚诱导率影响无显著差异。接种方式对成熟胚愈伤组织分化率影响极显著,胚切伤略带胚乳法>成熟胚刮碎法>完整胚法。诱导培养基附加适量添加物有利于愈伤组织分化,正交试验获得添加物最佳配比为100 mg·L-1谷氨酰胺+0.1 mg·L-1NAA+0.3 g·L-1肌醇+1 g·L-1脯氨酸+0.5 mg·L-1ABA。2,4-D浓度显著影响成熟胚愈伤组织分化率和成苗率,随2,4-D浓度升高,诱导率呈先升高后降低趋势,而分化率和成苗率均下降。分化培养基中6-BA对成苗率影响差异极显著,4 mg·L-1时达到最大;KT对愈伤组织分化要高于6-BA,但对成苗率影响明显低于6-BA。该体系所获冬小麦愈伤组织分化率和成苗率分别可达93.33%和66.64%。
译  名：
Establishment of tissue culture and regeneration system from mature embryos of winter wheat Dongnongdongmai1
作  者：
WANG Junhong;ZHENG Chengcheng;YU Jing;LU Qiuwei;WU Bing;ZHU Huijie;FENG Mingfang;MEI Lin;LI Weina;CHU Liyang;School of Life Sciences, Northeast Agricultural University;
关键词：
winter wheat(Triticum aestivum L.);;Dongnongdongmai1;;mature embryo;;tissue culture;;regeneration system
摘  要：
Dongnongdongmai1 is the first cultivated winter wheat which has been confirmed can live through the winter in the alpine region of Heilongjiang Province, setting up highly efficient and stable regeneration system of tissue culture has important significances to the improvement of varieties and functional genomics. The mature embryo of Dongnongdongmai1 was used as explants to explore the influence of callus induced, differentiation and plant regeneration, including the influence factors of medium,inoculation method, the concentration of additive combination, the concentrations of 2, 4-D, 6-BA and KT.The results showed that different type of medium had no significant affected on induction rate of wheat mature embryo. Different inoculation methods significantly effected on differentiation of mature embryo, with endosperm cut embryo slightly>scraped embryo>complete embryo. Added with appropriate amount of additives for inducing culture medium was conductive to callus differentiation and the orthogonal test got the best ratio of additives was 100 mg· L-1glutamine+0.1 mg· L-1NAA+0.3 g· L-1inositol+1 g· L-1proline+0.5 mg· L-1ABA. 2,4-D concentration significantly affected the differentiation rate and plant regeneration percentage of callus from mature embryo. With the increasing of 2, 4-D concentration, the induction rate was increased at first and then decreased, the differentiation rate and plant regeneration percentage all decreased. 6-BA in the differentiation medium had extremely significant effect on the differentiation rate and reached the peak at4 mg· L- 1; the differentiation of KT on callus was higher than 6-BA, but the effect on plant regeneration percentage was significantly lower than 6-BA. The differentiation rate and plant regeneration percentage of the system can reach 93.33% and 66.64%, respectively.

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