Research Progress on the Extraction,Preparation and Uses of Matrigel

Engelbreth-Holm-Swarm tumor%matrigel%basement membrane%extracellular matrix%organoid

The basement membrane is a specialized structure of extracellular matrix,which exists in most tissues.It not only provides supporting structure for various cell types such as epithelial cells,endothelial cells,nerve cells,muscle cells,but also plays an important role in maintaining the tissue structure and function.Matrigel is the soluble basement membrane preparation extracted from the mouse Engelbreth-Holm-Swarm(EHS)tumors rich in extracellular matrix protein.It contains almost all components of the basement membrane and can mimic its biological properties,which becomes the crucial biological material for many experimental models in life science research,especially the important media of organoids.In this review,the biological characteristics of matrigel,its extraction and preparation technology,and its main uses are summarized,and its potential application fields and values are discussed.Additionally,the current status of its production both at home and abroad and the existing problems are analyzed,and the future development trend of matrigel production is prospected.This study aims to provide reference for the development and application of matrigel in China.