中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 单播草地匍茎翦股颖根际联合固氮菌筛选及部分特性初探 甘肃农业大学学报 2009,44 (3) 112-116
Position: Home > Articles > Preliminary study on isolation and characteristics of associative nitrogen fixation bacteria from rhizosphere of single-sowing Agrostis stolonifera turf Journal of Gansu Agricultural University 2009,44 (3) 112-116

单播草地匍茎翦股颖根际联合固氮菌筛选及部分特性初探

作  者：
田宏;张德罡;姚拓
单  位：
甘肃农业大学草业学院;湖北省农科院畜牧兽医研究所
关键词：
匍茎翦股颖;联合固氮菌;固氮酶活性;乙炔还原法
摘  要：
从西安地区单播草坪匍茎翦股颖的根际分离到固氮菌株188株,通过气相色谱仪,利用乙炔还原法最终筛选出4株固氮酶活性较高的菌株进行固氮菌部分特性测定及初步对菌株进行鉴定.结果表明:4株菌株中,X4菌株固氮酶活性最高,C2H4达180.20 nmol.mL-1.h-1,与其他菌株相比差异极显著(P<0.01),X2菌株固氮酶活性最低,C2H4为39.32 nmol.mL-1.h-1;筛选出的4株菌株除X4菌株生长较慢外,其余菌株都生长迅速;X2、X3和X4菌株为革兰氏阳性菌株,X1为革兰氏阴性菌株;除X4为中性兼性厌氧性菌株外,其余均为碱性好氧性菌株.经生理生化实验初步鉴定,X1和X4分别属于Enterobacters和Staphylococcu属,X2和X3菌株属于Bacillus属.
译  名：
Preliminary study on isolation and characteristics of associative nitrogen fixation bacteria from rhizosphere of single-sowing Agrostis stolonifera turf
作  者：
TIAN Hong1,ZHANG De-gang2,YAO Tuo2(1.Institute of Poultry and Veterinarian,Hubei Academy of Agricultural Sciences,Wuhan 430209,China;2.College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China)
关键词：
Agrostis stolonifera;associative nitrogen fixation bacteria;nitrogenase activity;acetylene reduction assay
摘  要：
Total 188 associative nitrogen fixation bacteria strains were isolated from the rhizosphere of single-sowing Agrostis stolonifera turf in Xi'an.In which,4 strains(coded with X1,X2,X3 and X4) with higher nitrogenase activity were finally identified and studied by using acetylene reduction assay with gas chromatogrphy.The results showed that Nitrogenase activity of X4 was the highest,C2H4 of X4 reached to 180.20 nmol·mL-1·h-1,which was significantly higher than other strains.Nitrogenase activity of X2 was the lowest,C2H4 of which was 39.32 nmol·mL-1·h-1.All strains grew fast except X4,X2,X3 and X4 were gram positive bacteria while X1 was gram negative bacteria.X4 was neutral and semi-anaerobe while the other 3 strains were alkaline and aerobic.The identification results through physiological and physiochemical reactions showed that X1 and X4 belonged to Enterobacter and Staphylococcus respectively,X2 and X3 belonged to Bacillus.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46