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当前位置: 首页 > 文章 > 磁性Fe_3O_4/聚苯胺纳米纤维的制备及其固定漆酶研究 生物技术通报 2017,33 (3) 205-212
Position: Home > Articles > Preparation of Magnetic Fe_3O_4/Polyaniline Nanofiber for Laccase Immobilization Biotechnology Bulletin 2017,33 (3) 205-212

磁性Fe_3O_4/聚苯胺纳米纤维的制备及其固定漆酶研究

作  者：
刘友勋;王耀坤;闫明阳;黄娟
单  位：
新乡医学院基础医学院
关键词：
磁性纳米颗粒;聚苯胺;漆酶;固定化
摘  要：
合成优良的漆酶固定化载体有利于其进一步应用。通过将磁性纳米颗粒包埋在苯胺的聚合物中形成磁性Fe_3O_4/聚苯胺纳米纤维,作为漆酶固定化载体。透射电镜和红外图谱分别显示了载体的形态结构特征。不同比例的Fe_3O_4与苯胺对载体结构没有明显影响,但会影响酶的负载量。合成载体最大酶负载量为210 mg/g,固定漆酶后的载体导电性能发生变化。固定化漆酶最适pH从4偏移到3.5,在酸性pH范围保持较高的酶活性,最适温度为60℃;在50℃下孵育240 min,能保持约50%的酶活性,于4℃下保存30 d能保持约60%的酶活性;重复使用8次后还能保留70%的酶活性;结果证实了磁性Fe_3O_4/聚苯胺纳米纤维成功合成,对酶有较高的负载量。随着Fe_3O_4的比例增加,载体对漆酶的负载量却减少;漆酶与载体间存在有一定电子交流。固定化漆酶的最适pH向酸性偏移可能和聚苯胺的导电性有关,合成载体显示出良好的热稳定性、储存稳定性和重复使用稳定性,表明磁性Fe_3O_4/聚苯胺纳米纤维是一种优良的酶固定化载体,可以实现酶的高效固定化。
译  名：
Preparation of Magnetic Fe_3O_4/Polyaniline Nanofiber for Laccase Immobilization
作  者：
LIU You-xun;WANG Yao-kun;YAN Ming-yang;HUANG Juan;School of Basic Medical Sciences,Xinxiang Medical University;
关键词：
magnetic nanoparticle;;polyaniline;;laccase;;immobilization
摘  要：
It is the key to prepare a perfect carrier for enzyme immobilization for increasing enzyme loading,efficient use and reducingthe cost of enzyme application. Magnetical polyaniline nanocomposite was synthesized via mixing aniline solution and Fe_3O_4 nanoparticles andused as laccase immobilization carrier. The successful synthesis was confirmed by the results of TEM and FT-IR. Different proportions of Fe_3O_4 and aniline had no significant effect on the structure of the carrier,but the enzyme loading was affected. The maximum loading of the syntheticcarrier was 210 mg/g,and the conductive properties of carrier were changed after the laccase was immobilized on it. The optimum reaction pHand temperature for the immobilized laccase were 3.5 and 60℃,respectively. A slight shift of optimal pH was observed. Immobilized laccasekept 50% its activity after incubation at 50℃ for 240 min,and 60% of its activity after incubation at 4℃ for 30 d. Over a period of repeatedoperation,immobilized laccase still remained about 70% after 8 cycles. With the increase of the proportion of Fe_3O_4,the loading of the carrierreduced,and there was a certain electron exchange between laccase and the carrier. The immobilized laccase can be simply recovered fromthe reaction solution by a magnet. Immobilized laccase onto Fe_3O_4/polyaniline nanofiber shows high stabilities,easy recovery,and reusecapabilities,which demonstrated that Fe_3O_4/ polyaniline nanofiber is a kind of perfect carrier for enzyme immobilization.

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