中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 生长素、细胞分裂素对黑暗和ABA诱导蚕豆气孔关闭的抑制效应 作物学报 2008,34 (6) 1034-1041
Position: Home > Articles > The Inhibitory Effects of Auxin and Cytokinin on Dark- and ABA-induced Stomatal Closure in Broad Bean Acta Agronomica Sinica 2008,34 (6) 1034-1041

生长素、细胞分裂素对黑暗和ABA诱导蚕豆气孔关闭的抑制效应

作  者：
张蓓;佘小平;张广斌;孟朝妮;宋喜贵
单  位：
陕西师范大学生命科学学院
关键词：
生长素;细胞分裂素;一氧化氮;过氧化氢;暗;脱落酸;气孔关闭
摘  要：
以蚕豆(Vicia faba L.)为材料,借助表皮条实验和激光扫描共聚焦显微镜技术研究合成和天然生长素、细胞分裂素吲哚乙酸(IAA)、萘乙酸(NAA)、2,4-二氯苯氧乙酸(2,4-D)、玉米素(ZT)、激动素(KT)和6-苄基腺嘌呤(6-BA)对黑暗和脱落酸(ABA)诱导气孔关闭的效应及其机制。结果表明,黑暗和ABA均诱导气孔关闭,与NO清除剂羧基-2-苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物(c-PTIO)、牛血红蛋白(Hb)和NO合酶(NOS)抑制剂NG-N-L-精氨酸甲酯(L-NAME)以及过氧化氢(H2O2)清除剂抗坏血酸(AsA)、过氧化氢酶(CAT)和过氧化氢产生酶NADPH氧化酶抑制剂二苯基碘(DPI)一样,供试合成和天然生长素、细胞分裂素均显著逆转黑暗和ABA诱导的气孔关闭,暗示生长素、细胞分裂素可能通过降低NO和H2O2水平起作用。保卫细胞内源NO和H2O2检测结果显示,合成和天然生长素、细胞分裂素确实均显著降低暗和ABA诱导的NO和H2O2水平。考虑到IAA、ZT分别为天然生长素、细胞分裂素的典型代表而且供试生长素、细胞分裂素均包括了合成和天然种类,可以认为供试生长素、细胞分裂素逆转暗和ABA诱导的气孔关闭及降低NO、H2O2水平的作用可能是所有生长素类、细胞分裂素类植物生长物质均具备的效应。
译  名：
The Inhibitory Effects of Auxin and Cytokinin on Dark- and ABA-induced Stomatal Closure in Broad Bean
作  者：
ZHANG Bei, SHE Xiao-Ping, ZHANG Guang-Bin, MENG Zhao-Ni, and SONG Xi-Gui (College of Life Sciences, Shaanxi Normal University, Xi’an 71006, Shaanxi, China)
关键词：
Auxin; Cytokinin; Nitric oxide; Hydrogen peroxide; Dark; ABA; Stomatal closure
摘  要：
In the present studies, the effects and mechanisms of natural and synthetic auxin IAA, NAA, 2,4-D, cytokinin ZT, KT, 6-BA on dark- and ABA-induced stomatal closure were investigated by means of stomatal bioassay and using laser-scanning con- focal microscopy. Isolated epidermal strips of Vicia faba were incubated with IAA (10 μmol L-1), NAA (10 μmol L-1), 2,4-D (10 μmol L-1), ZT (0.1 μmol L-1), KT (0.2 μmol L-1), 6-BA (0.2 μmol L-1), NO scavenger c-PTIO (200 μmol L-1), Hb (100 μmol L-1), NOS inhibitor L-NAME (25 μmol L-1), H2O2 scavenger AsA (100 μmol L-1), CAT (100 U mL-1), inhibitor of H2O2-generating enzyme NADPH oxidase DPI (10 μmol L-1) for 3 h, in darkness or in light in the presence of ABA (1 μmol L-1), respectively. The results showed that auxin, cytokinin, as well as c-PTIO, Hb, L-NAME, AsA, CAT, and DPI, reversed dark- and ABA-induced stomatal closure significantly. Epidermal strips treated with auxin and cytokinin were loaded with NO-fluorescent dye DAF-2DA or H2O2-fluorescent dye H2DCF-DA. The results indicated that darkness and ABA could induce an intense DAF-2DA or H2DCF-DA fluorescence in guard cells. However, dark- and ABA-induced DAF-2DA and H2DCF-DA fluorescence were largely prevented by auxin and cytokinin tested. Similarly, the treatments of c-PTIO, Hb, L-NAME and AsA, CAT, DPI also substantially suppressed dark- and ABA-induced DAF-2DA and H2DCF-DA fluorescence, respectively. These results provide the evidence that auxin and cytokinin tested lessen assuredly NO and H2O2 levels induced by dark and ABA in guard cells. Consider- ing synthetic auxin, cytokinin NAA, 2,4-D, KT, 6-BA and natural IAA, ZT were used in the present work and IAA, ZT are repre- sentative of endogenous auxin and cytokinin respectively, the effects of auxin, cytokinin tested on dark- and ABA-induced stomatal closure and NO, H2O2 level can be attributed to an universal effect of auxin or cytokinin.

相似文章

PDF下载 PDF预览
导出引用
计量
文章访问数: 20
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46