Position: Home > Articles > Genome-wide Microarray Analysis of Helicoverpa armigera Larva Fed on Transgenic hpa1_(Xoo) Cotton Leaves
Scientia Agricultura Sinica
2010,43
(2)
313-321
转hpa1_(Xoo)基因棉花对棉铃虫全基因组转录谱的影响
作 者：
缪卫国;武淑文;宋从凤;汪瑜;龚晓崇;张亮;王金生
单 位：
博奥北京国家生物芯片公司;南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室
关键词：
转hpa1Xoo棉花;harpinXoo;Helicoverpa armigera;家蚕Oligo芯片;差异表达基因
摘 要：
【目的】明确转hpa1Xoo棉花抗虫性及其作用机制。【方法】采用生测法和芯片技术分析了转hpa1Xoo棉T-34的抗虫性和harpinXoo表达对棉铃虫全基因组转录谱的影响。【结果】饲喂T-34棉叶的棉铃虫发育明显迟缓,棉铃虫死亡率为90.95%,而饲喂其出发品种(Z35)棉叶的棉铃虫死亡率为12.20%。利用和棉铃虫同属鳞翅目的家蚕的23KOligo探针,以分别饲喂T-34和Z35叶片的棉铃虫RNA为模板,制作了棉铃虫Oligo表达谱芯片,获得了24280个EST数据,从中检测到了2927个基因。在T-34和Z35上饲喂的棉铃虫有872个差异表达基因,其中上调基因328个(ratio>2.0),下调基因544个(ratio<0.5)。872个差异表达基因主要涉及13个生物功能、96个代谢通路。【结论】转hpa1Xoo棉花对棉铃虫的抗性涉及棉铃虫蛋白酶分解和合成、ATP合成等多个生物代谢途径的改变。
译 名：
Genome-wide Microarray Analysis of Helicoverpa armigera Larva Fed on Transgenic hpa1_(Xoo) Cotton Leaves
作 者：
MIAO Wei-guo1,WU Shu-wen1,SONG Cong-feng1,WANG Yu1,GONG Xiao-chong1,ZHANG Liang2,WANG Jin-sheng1 (1College of Plant Protection,Nanjing Agricultural University/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing 210095; 2CapitalBio Corporation,Beijing 102206)
关键词：
transgenic cotton; harpinXoo; Helicoverpa armigera; 23K Oligo silkworm microarray; differentially expressed genes
摘 要：
【Objective】 The objective of this study was to confirm the resistance of transgenic hpa1Xoo cotton T-34 to cotton bollworm (Helicoverpa armigera) and its resistance mechanism. 【Method】 Bioassay and 23K Oligo silkworm microarray were used to analyze the insect resistance of T-34 and globe transcriptome profile of H. armigera kept by transgenic hpa1Xoo cotton leaves comparing to untransformed cotton Z35. 【Result】 The fresh leaves of T-34 and Z35 were utilized to keep H. armigera larva,and only T-34 made H. armigera larval development become slow rather than Z35. Microarray data indicated that 872 genes in H. armigera were totally deregulated by the comparison of T-34 vs Z35. Of 872 genes,328 genes were showed to be upregulated (ratio>2.0),and 544 genes were indicated to be downregulated (ratio<0.5). It was suggested that all differentially expressed genes might be classified and related to 13 biological functions,and involved in 96 biological pathways. 【Conclusion】 harpinXoo encoded by hpa1Xoo confers the resistance of transgenic hpa1Xoo cotton to H. armigera,and influences the multiple metabolism pathways in H. armigera larva.
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