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Nonwood Forest Research
2013,31
(1)
97-101
杜仲叶残渣酶预处理提取杜仲胶生产工艺
作 者：
任涛;李多伟;闫钊;侯莹莹;刘长霞
单 位：
陕西三原红旗实业有限公司;西北大学生命科学学院;陕西略阳县华瑞杜仲开发有限责任公司
关键词：
杜仲叶残渣;纤维素复合酶;总糖含量;杜仲胶
摘 要：
为了优化杜仲胶提取工艺,以酶解液中的总糖含量和酶解杜仲胶的提取率为试验考察指标,来确定纤维素复合酶预处理杜仲叶残渣的生产工艺条件.试验结果表明:纤维素复合酶预处理杜仲叶残渣的最佳工艺条件为pH值5.5,温度50℃,酶用量为1.6 mg/g,料液比为1∶15(g∶mL),酶解4 h.与非酶解原料相比,杜仲叶残渣经酶解预处理后胶提取率是未经酶解的1.49倍.表明纤维素复合酶可用于杜仲叶残渣提取杜仲胶的酶解预处理过程.
译 名：
Techniques of extracting gutta percha by enzymolysis pretreatment of leaf residue in Eucommia ulmoides Oliv.
单 位：
College of Life Sciences,Northwest University,Xi'an 710068,Shanxi,China%Sanyuan Red Flag Industrial Co.Ltd.,Xianyang 713800,Shanxi,China%Huarui Eucommia Development Co.Ltd.,Lueyang 724300,Shanxi,China
关键词：
Eucommia leaf residue%cellulose compound enzyme%total sugar content%gutta percha
摘 要：
In order to optimize extraction techniques of gutta percha,taking total sugar content and extraction rate of enzymatic gutta percha as experimental investigation indexes,the conditions of process were researched through enzymatic pretreatment of Eucommia leaf residue.The results show that the optimum conditions are pH 5.5,temperature of 50℃,enzyme dosage of 1.6 mg/g,ratio of solid to liquid of 1 ∶ 15(g ∶ mL),enzymolysis lasting for 4 hours.Compared with raw non-enzymolysis materials,the extraction rate of gutta percha from Eucommia leaf residue pretreated by enzymatic is 1.49 times of that of CK.So the cellulose compound enzyme can be used in the enzymolysis pretreatment process of gutta percha extracted from Eucommia leaf residue.
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