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当前位置: 首页 > 文章 > 米蛾体内Wolbachia的wsp基因序列测定与系统发育分析 昆虫学报 2005,48 (5) 169-172
Position: Home > Articles > Sequencing and phylogenetic analysis of the wsp gene of Wolbachia in Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) Acta Entomologica Sinica 2005,48 (5) 169-172

米蛾体内Wolbachia的wsp基因序列测定与系统发育分析

作  者：
丛斌;付海滨;王翠敏;戴秋慧
单  位：
现工作单位:大连出入境检验检疫局;害虫生物防治研究室 沈阳110161;现工作单位:深圳出入境检验检疫局;沈阳农业大学植物保护学院;害虫生物防治研究室
关键词：
Wolbachia;米蛾;wsp基因;赤眼蜂
摘  要：
Wolbachia是广泛分布于节肢动物体内的一类共生菌,它们参与多种调控寄主的生殖活动机制。通过对wsp基因的特异性扩增和测序,发现了Wolbachia在米蛾Corcyracephalonica(Stainton)体内的感染。利用所测序列和其他已发表的序列建立系统树,结果表明米蛾体内Wolbachia属于B大组的Pip类群,与其寄生物茧蜂及赤眼蜂中的Wolbachia各株系遗传距离相差较远。据此推测米蛾体内感染的Wolbachia不是由寄生物(茧蜂、赤眼蜂)水平传播所致。
译  名：
Sequencing and phylogenetic analysis of the wsp gene of Wolbachia in Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae)
作  者：
CONG Bin, FU Hai-Bin, WANG Cui-Min*, DAI Qiu-Hui ** (Laboratory for Bio-Control of Pest Insects, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China)
关键词：
Wolbachia; Corcyra cephalonica; wsp gene; Trichogramma spp.
摘  要：
Wolbachia is a common and widespread group of bacteria found in arthropods. These bacteria have evolved various mechanisms for manipulating reproduction of their hosts. The presence of Wolbachia in a laboratory population of Corcyra cephalonica (Stainton) was firstly identified based on amplification and sequencing part of the wsp gene. Aligned the obtained sequence with the published ones, the phylogenetic relationships among Wolbachia found in C. cephalonica and other insects were established. The Wolbachia found in C. cephalonica belong to the Pip group, far separated from the groups of Wolbachia found in braconid wasps and Trichogramma spp. It was so inferred that Wolbachia did not transmit horizontally between C. cephalonica and its parasitoids (Apanteles sp. and Trichogramma spp.).

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