中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 新疆石河子地区鸡皮刺螨形态学鉴定及其cox1基因比对分析 石河子大学学报(自然科学版) 2016,34 (1) 30-35
Position: Home > Articles > Morphology identification and comparison of cox1 gene of Dermanyssus gallinae in Shihezi,Xinjiang Journal of Shihezi University(Natural Science) 2016,34 (1) 30-35

新疆石河子地区鸡皮刺螨形态学鉴定及其cox1基因比对分析

作  者：
张星星;孟庆玲;乔军;刘田莉;贡莎莎;才学鹏;陈创夫
单  位：
石河子大学动物科技学院;中国农业科学院兰州兽医研究所
关键词：
鸡皮刺螨;形态学鉴定;分子生物学鉴定;cox1基因;石河子地区
摘  要：
为了确定新疆石河子地区流行的鸡螨虫的种类,采集本地区蛋鸡和斗鸡流行的螨样进行形态学观察鉴定,同时克隆并分析其线粒体DNA细胞色素C氧化酶亚基1(cox1)基因,并与Gen Bank登录的鸡皮刺螨参考序列对比分析同源性并构建系统进化树。初步形态学鉴定结果显示:该虫体呈长椭圆形,后部略宽,螯枝细而长;背面有1块背板,前部较宽,后缘平直;胸板拱桥形,宽大于长,板上刚毛2对;肛板圆三角形,肛孔位于肛板后部;雄螨的胸殖板和腹肛板相接形成全腹板,具有鸡皮刺螨的形态特征。分子生物学鉴定发现,本研究螨虫株cox1基因与法国和意大利鸡源鸡皮刺螨cox1基因序列同源性达99%,证实石河子地区流行的鸡螨虫为鸡皮刺螨,该螨虫与法国和意大利鸡源鸡皮刺螨具有较近的亲缘关系。
译  名：
Morphology identification and comparison of cox1 gene of Dermanyssus gallinae in Shihezi,Xinjiang
作  者：
ZHANG Xingxing;MENG Qingling;QIAO Jun;LIU Tianli;GONG Shasha;CAI Xuepeng;CHEN Chuangfu;Department of Animal Science and Technology, Shihezi University;Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;
关键词：
Dermanyssus gallinae;;classification and identification;;molecular identification;;cox1 gene;;the region of Shihezi
摘  要：
To clarify the species of chicken mites in the regicon of Shihezi, popular chicken mite samples in laying hens and fighting cocks in the region were collected and identified by morphological observation. Then mitochondrial DNA cytochrome C oxidase subunit 1(cox1) gene was cloned and sequenced. The sequences were analyzed by comparison with reference sequence of chicken mite deposited in Gen Bank, and homology and phylogenetic tree were analyzed. The morphological observation of the mites showed that collected chicken mites possess elongated oval, slightly wider rear, chelating branch thin and long; One piece back on the back, a wide front, the rear edge of the flat; chest plate arch shaped, more wide than long, on- board two pairs of bristles; anal plate round triangle, anal orifice plate located in the rear; sternal- genito plate and ventro- anal plate of male reproductive mites contact to form holoventral plate, which were preliminary identified as Dermanyssus gallinae. Identification of molecular biology based on cox1 gene indicated that the species of mites were Dermanyssus gallinae, which shared identities of99% for cox1 gene, with France and Italy chicken mite and had a closer relationship.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46