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Chinese Veterinary Science
2018
(10)
1290-1298
羟乙基化猴头菇多糖对小鼠树突状细胞和自然杀伤细胞相互作用的影响
作 者：
秦韬;罗洋;龙瑶;刘晓盼;丘富安;陈仕雄;宋玉龙;马玉芳;李健;任喆;黄一帆
关键词：
羟乙基化;树突状细胞;自然杀伤细胞;免疫因子
摘 要：
本研究旨在制备羟乙基化猴头菇多糖,考察羟乙基化猴头菇多糖对树突状细胞(DCs)与自然杀伤细胞(NKs)相互作用的影响。以环氧乙烷为羟乙基化试剂,对猴头菇多糖进行羟乙基化。并采用RT-PCR和Western-blot探索猴头菇多糖(HEP)和羟乙基化猴头菇多糖(HE-HEP)分别与DCs和NKs共同孵育后对细胞免疫因子mRNA表达的影响。结果表明,HEP和HE-HEP可以使IL-12、IL-15、IL-18、NF-κB、NKG2D、TNF-α、IFN-γ和GM-CSF的mRNA表达上调。31.25 g/mL、62.5 g/mL的HE-HEP对IL-12、IL-15、IL-18、NF-κB的mRNA表达方面的效果优于HEP,并且62.5 g/mL、125 g/mL的HE-HEP对NKG2D、TNF-α、IFN-γ和GM-CSF的mRNA表达方面的效果优于HEP。结果表明,羟乙基化修饰可以显著增强猴头菇多糖对DCs和NKs之间的相互作用,提高其免疫活性。
译 名：
Effects of HE-HEP on interaction between DCs and NKs in mice
作 者：
QIN Tao;LUO Yang;LONG Yao;LIU Xiao-pan;QIU Fu-an;CHEN Shi-xiong;SONG Yu-long;MA Yu-fang;LI Jian;REN Zhe;HUANG Yi-fan;Fujian Key Laboratory of Veterinary Chinese Medicine and Animal Health,Fujian Agriculture and Forestry University;
单 位：
QIN Tao%LUO Yang%LONG Yao%LIU Xiao-pan%QIU Fu-an%CHEN Shi-xiong%SONG Yu-long%MA Yu-fang%LI Jian%REN Zhe%HUANG Yi-fan%Fujian Key Laboratory of Veterinary Chinese Medicine and Animal Health,Fujian Agriculture and Forestry University
关键词：
hydroxyethyl;;dendritic cells;;natural killer cells;;immune factors
摘 要：
The purpose of this study was to prepare HE-HEP and to investigate the effect on the interaction of dendritic cells(DCs) and natural killer cells(NKs).Epoxyethane was used to hydroxyethane the HEP.RT-PCR and Western-blot methods were used to explore the effects of HEP and HE-HEP on the expression of cell immune factors m RNA.In result, HEP and HE-HEP could increase the expression of IL-12,IL-15,IL-18,NF-κB,NKG2 D,TNF-α,IFN-γ and GM-CSF m RNAs.The effect of 31.25 and 62.5 g/m L HE-HEP on expression of IL-12,IL-15,IL-18 and NF-κB m RNAs is better than HEP.Moreover,the effect of 62.5,125 g/m L HE-HEP on the expression of NKG2 D,TNF-α,IFN-γ and GM-CSF m RNAs is better than HEP.In conclusion,hydroxyethyl modification enhance significantly the effect of HEP on interaction between and DCs and NKs,and enhance their immune activity.
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