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当前位置: 首页 > 文章 > Mu转座子介导的玉米插入突变体的鉴定 分子植物育种 2011,09 (5) 56-62
Position: Home > Articles > Identification of the Mutator Insertional Mutants in Maize Molecular Plant Breeding 2011,09 (5) 56-62

Mu转座子介导的玉米插入突变体的鉴定

作  者：
陈果;李见坤;王国英;郑军
单  位：
中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程
关键词：
玉米;Mu转座子;蛋白磷酸酶;巢式PCR;突变体
摘  要：
选取5个对干旱胁迫响应的玉米蛋白磷酸酶基因,从Mu突变体库中定向筛选到这些基因共92个Mu转座子插入的候选突变体。在田间种植这些候选突变体的后代,每个12粒。利用巢式PCR对这些后代植株的目标插入位点进行鉴定,选择阳性植株与B73杂交或回交。在92个候选突变体中,有21个为可遗传的Mu转座子插入突变体;其中8个突变体的Mu插入位点在目的基因的5'非翻译区,10个在内含子区,3个在外显子区。通过连续回交和自交,获得了这些突变体的纯合株系。本研究为这些基因的功能分析奠定了材料基础。
译  名：
Identification of the Mutator Insertional Mutants in Maize
作  者：
Chen Guo Li Jiankun Wang Guoying Zheng Jun * Institute of Crop Sciences, National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081
关键词：
Maize (Zea Mays L.), Mutator transposon, Protein phosphatase, Nest PCR, Mutant
摘  要：
Ninty-two Mu-insertional candidate mutants of 5 protein phosphatase genes responsive to drought stress were screend from a maize Mu mutant library. Twelve progeny of every candidate mutant were grown and analyzed by nested PCR. The positive plants with Mu-insertion in the target gene were self-pollinated and back crossed with B73 in the field. The results showed that the Mu-insertion of 21 mutants were heritable and the flanking sequences of Mu-insertion were sequenced. Among the 21 heritable mutants, there were 8 insertions in the 5'-UTR, 10 in the introns and 3 in the exons. After several rounds of backcross and selfing, the homozygous Mu-Insertion lines were identified and they will be used for further functional analysis.

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