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当前位置: 首页 > 文章 > 玫瑰冠鸡IGF-1基因多态性与早期生长速度关系研究 石河子大学学报(自然科学版) 2007,25 (3) 319-322
Position: Home > Articles > Correlation Analysis between Polymorphism of the IGF-1 Gene and Body Growth in Rose Cockscomb Chickens Journal of Shihezi University(Natural Science) 2007,25 (3) 319-322

玫瑰冠鸡IGF-1基因多态性与早期生长速度关系研究

作  者：
李伟;李大全;李应生;孙凤霞;彭夏雨
单  位：
石河子大学动物科技学院
关键词：
玫瑰冠鸡;IGF-1基因;PCR-RFLP;DNA多态性;体重
摘  要：
以玫瑰冠类群鸡(玫瑰冠鸡)为材料,采用PCR-RFLP法检测了IGF-1基因5’调控区DNA序列多态性,并运用线性模型统计方法分析了多态性与初生重和12周龄体重的关系。结果显示:玫瑰冠鸡IGF-1基因5’调控区自然存在两种不同DNA序列,经PstI酶切后出现3种基因型("-/-"、"-/+"、"+/+"),基因型分布符合哈代-温伯格定律。各基因型个体的初生重、12周龄体重的最小二乘均存在"-/-">"+/-">"+/+"的趋势,且"-/-"型个体的12周龄体重显著高于"+/+"型个体(P<0.05)。
译  名：
Correlation Analysis between Polymorphism of the IGF-1 Gene and Body Growth in Rose Cockscomb Chickens
作  者：
LI Wei,LI Da-quan,LI Ying-sheng,SUN Feng-xia,PENG Xia-yu (College of Animal Science & Technology,Shihezi University,Shihezi,Xinjiang 832003,China)
关键词：
rose cockscomb chicken;IGF-I gene;PCR-RFLP;DNA polymorphism;body weight
摘  要：
The genetic variation of the 5'region of the IGF-I gene in rose cockscomb chickens was detected by PCR-RFLP.The relationship between DNA polymorphisms revealed by this method and bodyweight were ana1yzed with linear model.The results showed that there were three genotypes("-/-","-/+"and"+/+")after PCR products were cut by Pst I enzyme and the observed distribution of genotype was not different from the expected distribution under the assumption of the Hardy-Weinberg equilibrium for the IGF-I allele.There was a tendency that the genotype class Pst I(-/-)had the least square means of body weight for 12 weeks and birth in three genotype classes,while the genotype class Pst I(-/+)was intermediate between Pst I(-/-)and Pst I(+/+)class.Furthermore,Pst I(-/-)genotypes was associated with a significantly higher body weight for 12 weeks than the Pst I(+/+) genotypes(P<0.05).

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