家蚕丝素蛋白亲和多肽的筛选及验证
作 者：
郑胜男;雷芳;张青;毛传斌;杨明英
单 位：
浙江大学材料科学与工程学系
关键词：
噬菌体多肽库筛选;家蚕丝素蛋白;亲和多肽;特异性结合
摘 要：
家蚕丝素蛋白因其良好的生物相容性被广泛以修饰或基底材料的形式用于生物医用复合材料或器件,但与其他材料的结合性和相容性有待提高.通过噬菌体多肽库淘选技术筛选出家蚕丝素蛋白亲和肽有望解决这个问题.用噬菌体随机多肽库对家蚕丝素蛋白进行亲和筛选,获得多个家蚕丝素蛋白亲和多肽,其中亲和性最好的多肽序列为HVAWSWSWNNST,其出现的频率为42%.通过生物信息学软件RELIC/info验证了筛选过程的有效性.HVAWSWSWNNST多肽组在噬菌体捕获实验中的噬菌体回收数量最多且在噬菌体-酶联免疫吸附实验中具有最高的吸光度值,证明该多肽与丝素蛋白的结合力最强.该多肽的成功筛选,有望改善家蚕丝素蛋白与其他材料的结合性和相容性,并拓宽丝素蛋白在生物医学领域的应用.
作 者：
Zheng Shengnan;Lei Fang;Zhang Qing;Mao Chuanbin;Yang Mingying;School of Materials Science and Engineering,Zhejiang University;Research Institute of Applied Bioresources,College of Animal Science,Zhejiang University;
关键词：
Phage peptide library screening;;Silkworm fibroin;;Affinity peptide;;Specific binding
摘 要：
Silkworm fibroin is widely used in biomedical composite materials or devices as a modified or substrate material due to its good biocompatibility. But its binding ability and compatibility with other materials need to be improved. Affinity peptide screening for silkworm fibroin by phage peptide library panning technology is expected to solve this problem.In this study,a few affinity peptides for silkworm fibroin were obtained by affinity screening in phage random peptide library,among which the peptide HVAWSWSWNNST showed the best affinity with a frequency of 42%. Effectiveness of this screening process was verified by bioinformatic analysis. Since HVAWSWSWNNST peptide group had the biggest amount of recovered phages in the phage capture experiment,and the highest absorbance in phage-enzyme linked immunosorbent assays,it was suggested that its binding ability to silkworm fibroin was the strongest. The screened peptide is expected to improve the binding ability and compatibility of silk fibroin with other materials,and broaden its application in biomedical fields.
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