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Position: Home > Articles > Study on Stilbene from Roots of Polygonum multiglorum Thunb Antioxidant Activities in vitro FOOD SCIENCE 2007,28 (1) 313-317

何首乌中二苯乙烯苷的体外抗氧化研究

作  者:
吕丽爽
单  位:
南京师范大学金陵女子学院食品科学与工程系
关键词:
何首乌;二苯乙烯苷;抗氧化;清除自由基
摘  要:
从传统抗衰老中药何首乌中提取、分离、纯化得到不同组分:乙醇粗提物、大孔吸附树脂层析样品及二苯乙烯苷晶体,并运用HPLC-MS、NMR、X-衍射对二苯乙烯苷晶体进行了分析鉴定。对照白藜芦醇分别对三组分的抗氧化清除自由基性能进行了测定。包括还原能力、亚油酸体系抗氧化性能以及清除DPPH自由基、清除羟基自由基、清除超氧阴离子能力。结果表明:不同纯度的二苯乙烯苷均具有还原性,抗氧化及清除自由基性能,其中以二苯乙烯苷纯品最强。其半抑制率分别为:DPPHIC508.06μg/ml;羟基自由基IC502.75μg/ml;超氧阴离子IC500.57μg/ml;以上各指标均略高于白藜芦醇。
译  名:
Study on Stilbene from Roots of Polygonum multiglorum Thunb Antioxidant Activities in vitro
作  者:
LU Li-shuang (Department of Food Science and Technology, Jinling College, Nanjing Normal University, Nanjing 210097, China)
关键词:
Polygonum multiglorum Thunb;stilbene glycosides;antioxidant activity;free radical scavenging
摘  要:
Stilbene glycosides were isolated from the EtOH extract of the roots of Polygonum multiglorum Thunb. Three fractions were obtained: the crude EtOH extract, the fraction by macroporous resin and the purity crystal stilbene. The structures of crystal were determined by HPLC-MS, NMR and X- diffraction analysis. The antioxidant activities of three fraction of stilbene isolated were evaluated with different antioxidant tests, including reducing power, the lipid peroxides inhibition, free radical scavenging, superoxide anion radical scavenging and hydroxyl radical scavenging respectively in vitro. These various antioxidant activities were compared with resveratrol. The results indicated that the antioxidant activities of the three fractions are very good, and crystal stilbene is even more efficient: DPPH IC50 8.06μg/ml; hydroxyl radical scavenging IC50 2.75μg/ml and superoxide anion radical scavenging IC50 0.57μg/ml. All are belter than resveratrol.

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