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当前位置: 首页 > 文章 > 马铃薯X病毒的RT-PCR检测 园艺学报 2003,30 (6) 687-689
Position: Home > Articles > Detection of Potato Virus X by Reverse Transcription Polymerase Chain Reaction Acta Horticulturae Sinica 2003,30 (6) 687-689

马铃薯X病毒的RT-PCR检测

作  者：
孙琦;张春庆
单  位：
山东农业大学农学院
关键词：
马铃薯X病毒;病毒;检测;反转录PCR
摘  要：
通过对“一步法”RNA提取方法的改进,简化了提取程序,并保证了RNA的质量。根据马铃薯X病毒(PVX)基因序列设计合成了一对特异性引物,运用反转录PCR(RT-PCR)对PVX-RNA进行扩增,成功得到了与预期大小相一致的308 bp片段,而对照未得到任何产物,同时对反转录酶AMV用量做了不同的处理,得出AMV仅用1u仍能得到较好扩增的效果。从而建立了经济简便的PVX的RT-PCR检测体系,为PVX的防治、脱毒苗的检测提供有效手段。
译  名：
Detection of Potato Virus X by Reverse Transcription Polymerase Chain Reaction
作  者：
Sun Qi and Zhang Chunqing (Agronomy College, Shandong Agricultural University, Tai'an 271018, China)
关键词：
PVX (Potato virus X); Virus; Detection; Reverse transcription and polymerase chain reaction (RT-PCR)
摘  要：
Total RNA was extracted with an improved one-step method from tobacco leaves infected with potato virus X (PVX). The more quality of tested RNA was very good, which indicated that the improved method was very effective and simple. A pair of specific primers were designed and synthesized based on the homologous nucleotide sequence of different isolates of PVX by the reverse transcription and polymerase chain reaction (RT-PCR), a 308 bp DNA fragment was amplified successfully from PVX of Shandong isolate, and no fragment was obtained from control. With only 1U Reverse Transcriptase XL (AMV) , the amplified result were still very good. The results showed that an economical and convenient RT-PCR detection system of PVX was set up.

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