Optimization of Fermentation Conditions for the Production of Pectinase by Penicillium verruculosum TS63-9 and Its Application in Tobacco

HE Zhao-wei;XI Jia-qin;LI Yu-e;JIN Hong-shi;JIN Jiang-hua;SONG Ji-zhen;Jilin Tobacco Industrial Co.,Ltd.;Zhengzhou Tobacco Research Institute of CNTC;

Penicillium verruculosum Peyron.;;pectinase;;fermentation condition optimization;;cut tobacco;;smoking quality

Different conditions including carbon source, incubation time, temperature and p H for the production of pectinase by Penicillium verruculosum Peyron. TS63-9 had been studied. Then, the effects of crude enzyme of TS63-9 on cut tobacco were investigated from different areas. The results showed that, the optimium carbon source was 7.5 g / L tobacco stem powder,the optimium temperature and p H was 28 ℃ and 6.0. Under these conditions, the highest pectinase activity reached to 832.49 U / m L after incubating for 72 h at rotating speed 200 r / min. TS63-9 crude enzyme could decrease the pectin content significantly after treating several hours at given conditions. The smoking quality of treated cut tobacco was improved to a certain extent. Cell morphological characteristics of tobacco surface showed that the cell gaps became larger than untreated sample;and the ratios of cell gap increased greatly.