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Position: Home > Articles > Protoplasts Preparation Regeneration and Twice Mutagenesis of Strain Producing ε-polylysine FOOD SCIENCE 2008,29 (7) 277-280

ε-PL聚赖氨酸产生菌原生质体的制备与再生

作  者:
于雪骊;刘长江;种婷
单  位:
沈阳农业大学食品学院
关键词:
ε-聚赖氨酸;白色链霉菌;原生质体;诱变
摘  要:
本实验对ε-聚赖氨酸产生菌-白色链霉菌的原生质体制备、再生条件进行了研究,建立了白色链霉菌原生质体诱变体系。在此基础上,通过对原生质体进行脉冲光诱变,氦氖激光诱变,筛选得到一株高产菌株;通过双层平板实验证明比出发菌株抑菌圈的H/C大0.28~0.45cm;通过发酵证明比出发菌株产ε-聚赖氨酸的能力提高40%以上。
译  名:
Protoplasts Preparation Regeneration and Twice Mutagenesis of Strain Producing ε-polylysine
作  者:
YU Xue-li,LIU Chang-jiang,CHONG Ting (College of Food Science,Shenyang Agricultural University,Shenyang 110161, China)
关键词:
ε-polylysine;Streptomyces albulus;protoplast;mutation
摘  要:
To screen high ε-polylysine-producing mutants of Streptomyces albulus, the methods of protoplast preparation, regeneration and mutagenesis were established. Thereafter, the protoplasts of the original strain were treated by pulsed-ligh and He-Ne laser. The double layer culture medium test proved that the H/C value of mutation is 0.28~0.45 cm, bigger than that of the original strain. The fermentation result showed its ε-polylysine productivity is higher 40% than that of the original strain.

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