中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > UV-B处理对大豆异黄酮合成影响及GmUVR8基因克隆与表达分析 大豆科学 2022 (1) 28-35
Position: Home > Articles > Analysis of Isoflavone Synthesis Influenced by UV-B Treatment and Cloning of GmUVR8 Gene in Soybean Soybean Science 2022 (1) 28-35

UV-B处理对大豆异黄酮合成影响及GmUVR8基因克隆与表达分析

作  者：
张艺;于存浩;马晓红;姚陆铭;武天龙;王彪
单  位：
上海交通大学农业与生物学院;东北农业大学农学院
关键词：
大豆;UV-B;UVR8光受体;异黄酮;基因表达;基因克隆
摘  要：
为了探究UV-B辐射对大豆异黄酮合成调控的分子机理,本研究以UV-B辐射处理大豆V1期幼苗,采用HPLC和定量PCR方法分别测定处理前后各部位异黄酮含量和基因的表达,并克隆出GmUVR8光受体基因。发现UV-B辐射处理大豆V1期幼苗8 h后,叶中总异黄酮含量提高1.3倍,大豆苷元和染料木素分别提高15.8和16.5倍。大豆根、茎和叶中CHS和IFS基因对UV-B反应的时间和强度存在明显差异,CHS11在处理2 h后表达量增加38.4倍,IFS1和IFS2基因的最高表达量分别比处理前增加4.7和18.3倍。克隆出了大豆中编码UVR8光受体的GmUVR8a、GmUVR8b和GmUVR8c基因,其氨基酸序列与拟南芥AtUVR8的同源度为74%;GmUVR8a、GmUVR8b、GmUVR8c基因在叶中表达存在显著差异,以GmUVR8b表达量最高。结果表明在大豆中UV-B辐射可能通过多种UVR8光受体调控苯基丙酸类途径关键酶基因的表达,进而影响异黄酮的合成。
译  名：
Analysis of Isoflavone Synthesis Influenced by UV-B Treatment and Cloning of GmUVR8 Gene in Soybean
作  者：
ZHANG Yi;YU Cun-hao;MA Xiao-hong;YAO Lu-ming;WU Tian-long;WANG Biao;School of Agriculture and Biology, Shanghai Jiaotong University;College of Agriculture, Northeast Agricultural University;
关键词：
soybean;;UV-B;;UVR8 photoreceptor;;isoflavones;;gene expression;;gene clone
摘  要：
In order to explore molecular mechanism of soybean isoflavone synthesis influenced by UV-B radiation, isoflavone accumulation and gene expression levels in different parts of V1 stage, seedlings under UV-B treatment were investigated by HPLC and quantitative real-time PCR respectively, and genes encoding UV RESISTANCE LOCUS 8(UVR8) photoreceptor were also cloned. The accumulation of total isoflavones in leaves of soybean V1 stage was increased by 1.3 times under 8 h UV-B radiation, and daidzein, genistein improved by 15.8 and 16.5 times respectively. The transcripts of CHS and IFS exhibited obvious difference response to time after UV-B stress, and CHS11 was increased 38.4 times after 2 h treatment, and the highest expression levels of IFS1 and IFS2 increased by 4.7 and 18.3 times respectively. The coding sequences of UVR8 photoreceptor GmUVR8 a, GmUVR8 b and GmUVR8 c were cloned from soybean, the encoding protein had 74% homology in amino acids with AtUVR8, their expression levels in leaves showed significant difference, and GmUVR8 b displayed the highest transcripts among those genes. The results indicated that UV-B radiation might regulate synthesis of soybean isoflavones through multiple UVR8 light receptors which affect the expression of key enzymes in phenylpropanoid pathway.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46