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当前位置: 首页 > 文章 > 高效液相色谱法测定貉子肉中褪黑素残留量的研究 现代畜牧兽医 2016 (5) 1-4
Position: Home > Articles > Study on determination of the residual quantity of melatonin in nyctereutes procyonoides tissues using high performance liquid chromatography Modern Journal of Animal Husbandry and Veterinary Medicine 2016 (5) 1-4

高效液相色谱法测定貉子肉中褪黑素残留量的研究

作  者：
杨卫军;李曼;曹秀梅;林艳青;杜顺丰
单  位：
河北省秦皇岛市农产品质量安全监督检验中心
关键词：
高效液相色谱法;貉子肉;褪黑素;残留量
摘  要：
本研究建立一种检测貉子肉中褪黑素药物残留的高效液相色谱——荧光检测法。貉子肉经乙酸乙酯提取,旋转蒸发至干,溶解残留物,加入水饱和的正己烷去脂肪,Waters Oasis HLB柱净化。以20 mmol/L NaAc-HAc溶液和甲醇溶液为流动相,等度洗脱,检测波长:Ex=285nm,Em=345 nm。结果表明,褪黑素在0.1~100μg/L呈良好的线性关系,r均大于0.9999。方法的最低检出限为0.25μg/kg,定量限为1μg/kg。在1、5和10μg/kg的添加水平上,其回收率范围在72.0%~90.4%之间,相对标准偏差(RSD)为1.2%~2.4%。
译  名：
Study on determination of the residual quantity of melatonin in nyctereutes procyonoides tissues using high performance liquid chromatography
作  者：
Yang Weijun;Li Man;Cao Xiumei;Lin Yanqing;Du Shunfeng;Qinhuangdao Agricultural Product Quality Safety Supervision and Inspection Center;
关键词：
High performance liquid chromatography;;Nyctereutes procyonoides tissues;;Melatonin;;Residual quantity
摘  要：
A high performance liquid chromatography method has been developed for the simultaneous determination melatonin in nyctereutes procyonoides tissues muscle by fluorescence detector.Sample was extracted by ethyl acetate,the solution was purified with Waters Oasis HLB. Mobile phase of 20 mmol/L balanced solution(sodium acetate trihydrate-glacial acetic acid)-methanol was used with isocratic elution. The detection wavelength was Ex=285 nm, Em=345 nm.The results showed that the calibration curves of melatonin had good linearity relationship in the range from0.1 to 100 μg/L,and the correlation coefficient was greater than 0.9999. The average recovery rates of melatonin in muscle tissue were from 72.0% to 90.4% at three adding levels of 1, 5 and10 μg/kg for melatonin,and the values of relative standard deviation(RSD) for the average recovery rates of melatonin were from 1.2% to 2.4%,the limit of detection and limit of quantification were 0.25 μg/kg and 1 μg/kg, respectively.

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