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当前位置: 首页 > 文章 > 扁吻鱼和塔里木裂腹鱼线粒体COI基因片段的比较研究 水生态学杂志 2011,32 (1) 45-50
Position: Home > Articles > Comparative Study on Partial Mitochondrial COI Gene of Aspiorhynchus laticeps and Schizothorax biddulphi Journal of Hydroecology 2011,32 (1) 45-50

扁吻鱼和塔里木裂腹鱼线粒体COI基因片段的比较研究

作  者：
杨天燕;张人铭;郭焱;孟玮;海萨;马燕武
单  位：
新疆维吾尔自治区水产科学研究所
关键词：
扁吻鱼;塔里木裂腹鱼;线粒体COI基因
摘  要：
对扁吻鱼(Aspiorhynchus laticeps)和塔里木裂腹鱼(Schizothoraxbiddulphi)细胞色素c氧化酶亚基(COI)基因片段进行扩增。通过序列测定得到624bp的基因片段,无碱基的插入和缺失,A、T、G、C碱基中G含量最低,A+T含量高于G+C含量,与其它鱼类COI基因片段研究结果相一致。2个种间共检测到38处核苷酸替换,其中35处碱基转换,3处碱基颠换,蛋白质编码基因上的核苷酸替代主要是密码子第3位点上的同义替换。在编码的208个氨基酸中,仅发生2处氨基酸替代,替代率为0.96%。采用Kimura-2法计算种间平均遗传距离为0.06,以鲤作外群构建NJ系统树,扁吻鱼和塔里木裂腹鱼亲缘关系较近,而与新疆裸重唇鱼、斑重唇鱼亲缘关系较远。
译  名：
Comparative Study on Partial Mitochondrial COI Gene of Aspiorhynchus laticeps and Schizothorax biddulphi
作  者：
YANG Tian-yan,ZHANG Ren-ming,GUO Yan,MENG Wei,HAI Sa,MA Yan-wu(Xinjiang Fisheries Research Institute,Urumqi 830000,China)
关键词：
Aspiorhynchus laticeps;Schizothorax biddulphi;mtDNA COI gene
摘  要：
Mitochondrial cytochrome oxidase subunit I(COI)fragments of Aspiorhynchus laticeps and Schizothorax biddulphi were amplified.By sequencing,the sequence of 624 bp nucleotide was obtained,and no insertion/deletion was observed.Base G content was the lowest of all,and the content of A+T was higher than that of G+C,which was similar to the results in other fishes.38 nucleotide substitutions were checked between the two species which included 35 transitions and 3 transversions,and most of them were synonymous transitions at the third codon positions in the protein-coding genes.There were only two amino acid substitution in encoded 208 amino acids,and the substitution ratio was 0.96%.The average genetic distance by Kimura-2 method between A.laticeps and S.biddulphi was 0.06.Cyprinus cyprio was chosen as an out-group,and the NJ clustering tree indicated that A.laticeps and S.biddulphi had the closest relationship.

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