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Chinese Journal of Oil Crop Sciences
2000,22
(4)
花生丛枝病病原的血清学研究
作 者：
杨健源;梁志慧;唐伟文
单 位：
华南农业大学资环院;广东省农科院植保所
关键词：
花生丛枝病;血清学;植原体
摘 要：
本研究首次制备了花生丛枝病 ( PWB)植原体的专化性鼠腹水抗体 ,效价为 1∶ 1 2 80 ,比较了 5种间接 ELISA方法检测植原体的效果 ,并对 6种植原体病原提纯物进行了血清学研究。结果表明 ,鼠腹水抗体除与 PWB有血清学反应外 ,还与豇豆丛枝病原 ( Co WB)具有一定的血清学关系 ,而与 TWB、Ch WB、SWB和BWB无血清学关系。
译 名：
Serological aspects of phytoplasma associated with peanut witches' broom disease
作 者：
YANG Jian yuan 1, LIANG Zhi hui 2, TANG Wei wen 2 ( 1. Research Institute of Plant Protection, Guangdong Academy of Agr Sci, Guangzhou 510640, China; 2. College of Natural Resources and Environments, South China Agri Univ, Guangzhou 510640, China
关键词：
Peanut witches' broom disease (PWB); Serology; Phytoplasma
摘 要：
Specific mouse ascites fluid against phytoplasma associated with peanut witches' broom disease (PWB) was obtained for the first time and it gave a titer of 1∶1280 by common indirect ELISA. The sensitivities of 5 indirect ELISA methods were compared to detect phytoplasma in terms of dilution. Both of indirect PVP ELISA and SDS double immunodiffusion test were used to assess the serological relationships for 6 kinds of phytoplasmas. The result showed that both PWB and CoWB (Cowpea witches' broom disease) showed a positive reaction with the PWB mouse ascites fluid, while TWB, SWB, ChWB, BWB showed negative reaction.
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