单 位：
江南大学生物工程学院,工业生物技术教育部重点实验室;江南大学生物工程学院
关键词：
镰刀霉菌;色素;Plackett-Burman设计;响应面;培养基
摘 要：
用Plackett-Burman试验设计和响应面法(response surface methodology,RSM)对镰刀霉菌JN158产色素的发酵培养基7种营养物质配比进行优化。结果表明:蔗糖、花生饼粉和FeSO4·7H2O的添加量显著影响色素产量;用最陡爬坡试验使这3个显著因素的添加量接近最大响应区域,优化后,镰刀霉菌产色素的培养基添加量分别为:蔗糖30.18 g/L、花生饼粉4.86 g/L、K2HPO4·3H2O 1 g/L、NaCl 0.5 g/L、MgSO4·7H2O 0.5 g/L、CaCl20.5 g/L、FeSO4·7H2O 0.0122 g/L,预测的最大色素产量是1.268 g/L,验证实验得到的色素产量为(1.252±0.011)g/L,与预测值相近,优化后色素的产量是以前的2.9倍。
译 名：
Optimization of Medium Composition for Pigment Production by Fusarium sp. JN158 with Response Surface Methodology
作 者：
WANG Yong-wei;LIAO Xiang-ru;LI Xun-hang;CAI Yu-jie;Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University;
关键词：
Fusarium;;pigment;;Plackett-Burman design;;response surface methodology;;medium
摘 要：
Plackett-Burman design and response surface methodology(RSM) were applied to optimize 7 components in the medium for pigment production by Fusarium sp.JN158. The results showed that the yield of pigment was mainly influenced by the concentrations of glucose, peanut meal and FeSO 4 ·7H 2 O. With central composite design and RSM, the optimal medium composition and concentrations was determined to be sucrose(30.18 g/L), peanut meal(4.86 g/L), K 2 HPO 4 ·3H 2 O(1 g/L),NaCl(0.5 g/L), MgSO 4 ·7H 2 O(0.5 g/L), CaCl 2(0.5 g/L), and FeSO 4 ·7H 2 O(0.0122 g/L). The experimental yield of pigment produced by Fusarium sp. JN158 in the optimized medium was(1.252±0.011) g/L, which was close to the predicted value of 1.268 g/L.
