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当前位置: 首页 > 文章 > 藏猪与雅南猪IGF-I基因的克隆与序列分析 浙江农业学报 2015,27 (8) 1324-1330
Position: Home > Articles > Cloning and sequence analysis of IGF-I gene in Tibetan pig and Yanan pig Acta Agriculturae Zhejiangensis 2015,27 (8) 1324-1330

藏猪与雅南猪IGF-I基因的克隆与序列分析

作  者：
李伟;张飞燕;宋婷婷;谷笑笑;刘红露;潘大敏;韩书饼;潘康成
单  位：
四川农业大学动物医学院动物微生态研究中心
关键词：
藏猪;雅南猪;IGF-I基因;序列分析
摘  要：
为研究IGF-I基因对藏猪、雅南猪生长发育的影响,以Gen Bank中猪IGF-I基因序列(登录号:DQ121132.1)设计引物,提取藏猪、雅南猪肝脏组织总RNA,应用RT-PCR技术克隆出IGF-I基因,分别将藏猪、雅南猪IGF-I基因克隆到PMD19-T载体上,构建重组质粒p MD19-T-IGF-I,经PCR鉴定、测序分析得该基因大小为612 bp,包含一个完整的ORF,该ORF共有462个碱基,编码153个氨基酸;所测得的藏猪和雅南猪基因序列与Muller等报道的猪IGF-I基因编码序列高度同源,序列比对发现,藏猪的同源性为100%,雅南猪的为99.61%;藏猪IGF-I与雅南猪IGF-I基因序列的同源性为99.61%;雅南猪在258 bp处发生A→G突变,但其编码的氨基酸未发生改变。
译  名：
Cloning and sequence analysis of IGF-I gene in Tibetan pig and Yanan pig
作  者：
LI Wei;Zhang Fei-yan;SONG Ting-ting;GU Xiao-xiao;LIU Hong-lu;PAN Da-min;HAN Shu-bing;PAN Kang-cheng;Animal Microecology Institute,College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;
关键词：
Tibetan pig;;Yanan pig;;IGF-I gene;;sequence analysis
摘  要：
To study the impact of IGF-I gene on the growth of Tibetan pig and Yanan pig,a pair of primers was designed by sus scrofa IGF-I gene sequence from Gen Bank( Accession number: DQ121132. 1),the total RNAs were extracted by using Trizol from the livers of Tibetan pig and Yanan pig and used as template to amplify IGF-I gene by RT-PCR,the amplified fragments were cloned into p MD19-T vector and the recombinant plasmids p MD19T-IGF-I were constructed and sequenced. The sequencing results indicated that the IGF-I gene consisted of 612 nucleotides,containing a complete ORF of 462 bp ecoding 153 amino acids. The two IGF-I gene sequences shared high homology with the porcine IGF-I gene reported by Muller,et al.,which had an identity of 100% in Tibetan pig and 99. 61% in Yanan pig. It shared 99. 61% homology between the Tibetan pig and Yanan pig IGF-I gene. An A→G mutation occurred in the 258 bp of Yanan pig,but the corresponding amino acid was not changed.

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