当前位置: 首页 > 文章 > 联合PCR诊断大熊猫犬瘟热和犬冠状病毒的混合感染 吉林农业大学学报 2003,25 (1) 94-96
Position: Home > Articles > Diagnosis of Complex Infection of Canine Distemper Virus (CDV) and Canine Coronavirus (CCV) to Giant Panda by Multi-PCR Journal of Jilin Agricultural University 2003,25 (1) 94-96

联合PCR诊断大熊猫犬瘟热和犬冠状病毒的混合感染

作  者:
高凤山;胡桂学;夏咸柱;柏亚铎;余春;乔军
单  位:
吉林农业大学动物科技学院;解放军军需大学犬病研究中心
关键词:
大熊猫;犬瘟热;犬冠状病毒
摘  要:
采用一步法提取大熊猫肝脏细胞总RNA,经反转录,选择CDV和CCV联合PCR引物,一次性扩增出CDV和CCV目的片段,进一步证明了大熊猫同时感染了CDV和CCV。
译  名:
Diagnosis of Complex Infection of Canine Distemper Virus (CDV) and Canine Coronavirus (CCV) to Giant Panda by Multi-PCR
作  者:
GAO Fengshan1, HU Guixue1, XIA Xianzhu2, BAI Yaduo1, YU Chun2, QIAO Jun2(1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118,China; 2.Institute of Military,Quartermaster University of PLA,Changchun, Jilin 130062,China)
关键词:
pandas; canine distemper; canine coronavirus
摘  要:
Total RNA was extracted from the liver of two dead giant pandas. After reverse transcription, the obtained DNA was amplified by PCR with the CDV and CCR primers. The specific fragments of CDR and CCR were successfully identified from the amplified products, indicating that the dead giant pandas were infected with both CDR and CCR.

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