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当前位置: 首页 > 文章 > 臭氧减菌化处理罗非鱼片冰温贮藏过程中蛋白质生化特性的变化 水产学报 2015,39 (10) 1569-1576
Position: Home > Articles > Changes in biochemical properties of muscle protein from Nile tilapia fillets sterilized by ozone treatment during iced storage Journal of Fisheries of China 2015,39 (10) 1569-1576

臭氧减菌化处理罗非鱼片冰温贮藏过程中蛋白质生化特性的变化

作  者：
张红杰;赵永强;李来好;杨贤庆;郝淑贤;魏涯;岑剑伟;李娜
单  位：
中国水产科学研究院南海水产研究所
关键词：
罗非鱼片;臭氧减菌化;冰温贮藏;生化特性
摘  要：
为研究臭氧减菌化处理罗非鱼片在冰温贮藏过程中蛋白质生化特性变化规律,本研究对冰温贮藏过程中经臭氧处理罗非鱼片肌原纤维蛋白盐溶性、Ca2+-ATPase活性、表面疏水性和巯基及羰基含量变化进行了评价。结果显示,在冰温贮藏过程中,臭氧处理组和对照组罗非鱼片蛋白质均发生了不同程度的变性,表现为随贮藏时间的延长,肌原纤维蛋白盐溶性、Ca2+-ATPase活性及巯基含量均呈下降趋势,而肌动球蛋白表面疏水性和羰基含量则呈上升趋势;冰温贮藏20 d后,臭氧处理组罗非鱼片肌原纤维蛋白盐溶性、Ca2+-ATPase活性和巯基含量分别下降了76.57%、89.76%和66.72%,而对照组则分别下降了69.05%、86.45%和62.29%;另一方面,臭氧处理组罗非鱼片肌动球蛋白表面疏水性和羰基含量分别上升了111.75%和76.46%,对照组则分别上升了104.77%和58.23%。在同一贮藏时间臭氧处理组罗非鱼片肌原纤维蛋白盐溶性、Ca2+-ATPase活性及巯基含量较对照组低,而肌动球蛋白表面疏水性和羰基含量较对照组高。臭氧水的氧化性及处理过程中产生的ROS加速了冰温贮藏过程中罗非鱼肌肉蛋白质的变性。
译  名：
Changes in biochemical properties of muscle protein from Nile tilapia fillets sterilized by ozone treatment during iced storage
作  者：
ZHANG Hongjie;ZHAO Yongqiang;LI Laihao;YANG Xianqing;HAO Shuxian;WEI Ya;CEN Jianwei;LI Na;Key Laboratory of Aquatic Product Processing,Ministry of Agriculture;National R&D Center for Aquatic Product Processing;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;College of Food Science and Technology,Shanghai Ocean University;
关键词：
tilapia fillets;;sterilized by ozone treatment;;iced storage;;biochemical properties
摘  要：
Nile tilapia( Oreochromis niloticus) is one of the widely cultured fish species in the world,and has been known as a good rawmaterial in seafood processing field. In this paper,the changes of biochemical properties of muscle protein from Nile tilapia fillets sterilized by ozone treatment during iced storage were investigated by changes evaluation of myofibrillar protein salt solubility,Ca2 +-ATPase activity,surface hydrophobicity of actomyosin,total sulfhydryl content of actomyosin and carbonyl content. The results showed that different degrees of protein degeneration of ozone-water pretreatment group and control group Nile tilapia fillets all occurred during iced storage. The myofibrillar protein salt solubility,Ca2 +-ATPase activity and total sulfhydryl content all decreased as the storage time was extended. Moreover,both surface hydrophobicity of actomyosin and carbonyl content increased during iced storage. After being stored for 20 days,the myofibrillar protein salt solubility,Ca2 +-ATPase activity and total sulfhydryl content of ozone-water pretreatment group decreased by 76. 57%,89. 76% and 66. 72% respectively,while those of control group declined by 69. 05%,86. 45% and 62. 29%. On the other hand,surface hydrophobicity of actomyosin and carbonyl content of ozone-water pretreatment group increased by 111. 75% and 76. 46% respectively,while those of control group rose by 104. 77% and 58. 23%. At the same storage time,the myofibrillar protein salt solubility,Ca2 +-ATPase activity and total sulfhydryl content of ozone-water pretreatment group were lower than those of control group,and both surface hydrophobicity of actomyosin and carbonyl content were higher than those of control group. The research results indicated that the protein denaturation was promoted by the oxidation of ozone-water and ROS generated from ozone-water during storage period.

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