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当前位置: 首页 > 文章 > 条浒苔多糖的酶法辅助提取及其抗氧化活性 水产科学 2013,32 (4) 187-191
Position: Home > Articles > Enzymatic-assisted Extraction Process and Antioxidant Activity of Polysaccharides from Green AlgaEnteromorpha clathrata Fisheries Science 2013,32 (4) 187-191

条浒苔多糖的酶法辅助提取及其抗氧化活性

作  者：
盘赛昆;朱强;王淑军;臧莹
单  位：
淮海工学院食品工程学院;江苏省海洋生物技术重点建设实验室
关键词：
条浒苔;多糖;酶法辅助提取;抗氧化活性
摘  要：
以多糖提取率为指标,通过单因素试验确定提取条件,采用均匀设计优化纤维素酶辅助提取,采用普鲁士蓝反应法及Fenton体系测定条浒苔多糖的还原力及对羟自由基的清除活性。试验结果表明,条浒苔多糖的提取条件为料液比1∶20,pH 9.0,温度80℃,时间6h;纤维素酶辅助提取条件为温度25℃,pH 3.0,时间6.5h,料液比1∶5,加酶量0.5%。条浒苔多糖的提取工艺为经酶处理后调整料液比至1∶20,pH及温度调整至9.0及80℃,提取6h,在此条件下多糖提取率为31.05%～37.41%。条浒苔多糖的还原力及羟自由基清除率EC50分别为32.79mg/mL和4.10mg/mL。
译  名：
Enzymatic-assisted Extraction Process and Antioxidant Activity of Polysaccharides from Green AlgaEnteromorpha clathrata
作  者：
PAN Sai-kun 1,2,ZHU Qiang 1,2,WANG Shu-jun 1,2,ZANG Ying 2(1.Jiangsu Key Laboratory of Marine Biotechnology,Lianyungang 222005,China;2.School of Food Engineering,Huaihai Institute of Technology,Lianyungang 222005,China)
关键词：
Enteromorpha clathrata;polysaccharide;enzymatic-assisted extraction;antioxidant activity
摘  要：
The extraction process of polysaccharides from green alga Enteromorpha clathrata were investigated using uniform design and the antioxidant activity of the polysaccharides was tested.It was found that the optimal hot water extraction was performed under the conditions of ratio of solid to liquid= 1∶20,pH 9.0,80 ℃,and extracting period of 6h.The cellulase-assisted extraction was carried out under the ratio of solid to liquid=1∶5,pH 3.0,25℃,treating period of 6.5h,and 0.5%enzyme.The samples were treated with cellulase,and then extracted by hot water under the above conditions,with the extraction rate of 38.49%.The antioxidant test revealed that the polysaccharides showed certain reduction power and scavenging activity for hydroxyl free radicals.The EC50of the reduction power was found to be 32.79mg/mL and scavenging activity for hydroxyl free radicals 4.10mg/mL.

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